LOCUS       sequence-81970-e        1149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of HA tagged HIF1alpha with a deletion of the 
            N-terminal activation domain and R27G mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Koshiji M, Kageyama Y, Pete EA, Horikawa I, Barrett JC, Huang LE
  TITLE     HIF-1alpha induces cell cycle arrest by functionally counteracting 
            Myc.
  JOURNAL   EMBO J. 2004 May 5;23(9):1949-56. Epub 2004 Apr 8.
  PUBMED    15071503
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2004-05-5"; volume: "23(9)"; pages: "1949-56. Epub 2004 Apr 
            8"
COMMENT     Sequence Label: p(HA)HIF1alpha(401delta603)R27G
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tagcatttag gtgacactat agaatagggc cctctagata tatgcatatc tttctatatt
       61 atgtaaatgg ctttacccat ttaaataata aaccatacag catttaagaa tcattattat
      121 atgattaaca atgtcatgtt ccaggtttaa caatttcata ggccaaaaaa aatttcttct
      181 taaaaactag ttttataaac gcagaatata ttccatgagt aactgctggt attttttaag
      241 aaaatatatt gtgcaattgt ggctaccacg tactgctggc aaagcattat tatttatgta
      301 aaatgtgaaa aaaaaggtgt aaaaattttt caactgccta tgatcatgat gaaaggttac
      361 tgccttctta caaaaattat attggcatct tcttaaaaat aattcgaaaa agggataaac
      421 tccctagcca aaaataaata aataaaaagg tgcatttttt aaaatgatgc tactgcaatg
      481 caatggttta aataccaaaa aactgagaaa atgagctgtc tgtgatccag cattaaagaa
      541 catactaaaa aagagcatta atgtaaatta agtagaaagg ggatcaaaaa ttgaactaac
      601 caagtttgtg cagtattgta gccaggcttc taaaattaga tgtagaaaat ataaatagac
      661 tgctttaggt agtgagccac cagtgtccaa aaaaaggaat gaaattaaga aaaagctcag
      721 ttaacttgat ccaaagctct gagtaattct tcaccctgca gtaggtttct gctgccttgt
      781 ataggagcat taacttcaca atcataactg gtcagctgtg gtaatccact ttcatccatt
      841 gattgcccca gcagtctaca tgctaaatca gagggtatta aaataattgt cttttgctcc
      901 attccattct gttcactaga tttgcatcct tttacacgtt tccaagaaag tgatgtagta
      961 gctgcatgat cgtctggctg ctgtaataat gttccaattc ctactgcttg aaaaagtgaa
     1021 ccatcatgtt ccatttttcg ctttctctga gcattctgca aagctagtat ctttggattt
     1081 agttcttcct caggaactgt agttctttga ctcaaagcga cagataacac gttagggctt
     1141 cttggatga
//
