LOCUS       sequence-81975-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of Arch-GFP for optogenetic inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Chow BY, Han X, Dobry AS, Qian X, Chuong AS, Li M, Henninger MA, 
            Belfort GM, Lin Y, Monahan PE, Boyden ES
  TITLE     High-performance genetically targetable optical neural silencing by 
            light-driven proton pumps.
  JOURNAL   Nature. 2010 Jan 7. 463(7277):98-102.
  PUBMED    20054397
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jan 7. 463(7277):98-102."
COMMENT     Sequence Label: FCK-Arch-GFP
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..818)
                     /codon_start=1
                     /product="light-driven proton pump archaerhodopsin-3 from 
                     Halorubrum sodomense (Chow et al., 2010)"
                     /label=Arch
                     /translation="MDPIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVPGIASAAYLSMFFGIGLTEVTVGGEMLDIYYARYADWLFTTPLLL
                     LDLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYFLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGADVSAAD"
     regulatory      815..824
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcact gctactaccg gtcggtcggc ggcactgaca
       61 tcggcaccgg cactgggttc tggtgcctcg gtgtcgccca gaatagcccg ggatctcaac
      121 aggataaagc caaagccgac cttggcagtc acgtccaaca ccataaacag cagagtttcg
      181 atgcccaggc ccaccacgcc agcgccctca gtgcctatga tccacaggat agggtaagcg
      241 gtccacagca ccaagaccag agctgtcagg gtgttaaagg tagatgccac ctcggggccc
      301 cgctcctttg cagcagatcg cagggatgta gccagaaaat agagcaccac tatcatgcaa
      361 attgtagaga acaaccacca actgtatctg gctatggccg tgtggctcaa ggctccgatg
      421 aggccagtga cgatcatcag ggcgtccaca cccaccaggg tgccgatggt cacccgatcc
      481 accttagcga gaagggccag atccagcagc agaagtgggg tggtaaacag ccagtcggcg
      541 tacctggcat aatagatatc caacatttcg cccccgacgg tcacctcagt aagcccgata
      601 ccaaagaaca tagacagata tgcggcggat gcgattccgg gcaccaggat agtcacagcg
      661 taatattccc gggcatcctt atcggtgact ccccatccgc ggaccagaaa gtagaaggtt
      721 ccaatcagca tcagcagagt gccgatgccc agccacagag tttcaggtct gccgtcaccc
      781 agcaggtcgt aaccagcctg cagagcgatg gggtccatgg tggcggatcc cccgctgccc
      841 ccagaactag gggccactcg cctgcccgtg ctcctgagtg caaacggaga accggcttga
      901 ctgacgagct tggggcttct gagcagggca ctgtggctgc tcacagcctc gcgag
//
