LOCUS       sequence-81978-e        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Jin J, Ang XL, Shirogane T, Wade Harper J
  TITLE     Identification of substrates for F-box proteins.
  JOURNAL   Methods Enzymol. 2005;399:287-309. doi: 
            10.1016/S0076-6879(05)99020-4.
  PUBMED    16338364
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2005"; volume: "399:287-309. doi"; pages: " 
            10.1016/S0076-6879(05)99020-4"
COMMENT     Sequence Label: pcDNA3-DN-hCUL1-FLAG
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(54..77)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcatttag gtgacactat agaatagggc cctctagatg catgctcgag tcacttgtca
       61 tcgtcatcct tgtagtcgac aaccatcact tgattgagtg tgtcttctag ttctgcctcc
      121 tctgggttct tggaactttt cttcaacaag gagtcacagt atcgagccag caactcaggg
      181 gatttactgg atgattgggc catcttggta accgcgttgt tgtttatgaa gcgaccacaa
      241 gccttatcaa gagcagccac aaagccagcg tcattgttga atgcagacat taccagggca
      301 ttgtattttt tatgaacatc aagcactgtc tgtacataca ttttggggtc atttaaagca
      361 gcttctccac acttttcaat ggctgcaaga ccctgattat gaatgtgtgt ctccaacagt
      421 tttttcaatt ctcctaggcc atcctggatt ctagatacaa gattatacat gcgtcccaaa
      481 tcttcatttt tgtcagcatc caataaattc tgaaattctg tgtggaaaat ttccaagtgt
      541 ttttcaatga ggacttgttc acatttcctt gctaattcat cttgtgtgct ttcatgaagg
      601 taaacctgaa ctcttcgttg ttcctcaagc agacgagcct ctgccttttt catatattca
      661 gtaactgggt tctgctgcaa gaattcagta ctctctctgg tataaaatct ctctgtgtca
      721 gccaaaaatt gagattcaaa ggattcttta tacactgtta acgtagggcc ctttgcaaat
      781 gcatcatctt cattcagccc caattccacg taagactgta caactccact aatcaatctt
      841 gtattgatgg tttcaccatt cctttccttt tcaatcagct ttaaaacagc atttgttacc
      901 tgtttattca gtggcctgaa cagacagtct ctccaagtca ccaatgcaag cgaatagatt
      961 tcatatattc cttttcgtcc ttcgtcacat tcacggcgaa c
//
