LOCUS       sequence-81992-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Marx CJ, Lidstrom ME
  TITLE     Broad-host-range cre-lox system for antibiotic marker recycling in 
            gram-negative bacteria.
  JOURNAL   Biotechniques. 2002 Nov;33(5):1062-7.
  PUBMED    12449384
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2002-11"; volume: "33(5)"; pages: "1062-7"
COMMENT     Sequence Label: pCM184
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(31..64)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(187..206)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(271..290)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 attggtacca tggatgcata tggcggccgc ataacttcgt atagcataca ttatacgaag
       61 ttatctagac ctgcaggggg gggggggaaa gccacgttgt gtctcaaaat ctctgatgtt
      121 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
      181 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat
      241 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
      301 aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga
      361 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
      421 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
      481 ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg
      541 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
      601 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
      661 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
      721 ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca
      781 ctcatggtga tttctcactt
//
