LOCUS       sequence-81993-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The oriP/EBV nuclear antigen (EBNA)-1 elements of Epstein-Barr virus
            is fused in frame with the luciferase in the herpes simplex virus 
            type-1 amplicon vector, provides prolonged transgene expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Sia KC, Chong WK, Ho IA, Yulyana Y, Endaya B, Huynh H, Lam PY
  TITLE     Hybrid herpes simplex virus/Epstein-Barr virus amplicon viral 
            vectors confer enhanced transgene expression in primary human tumors
            and human bone marrow-derived mesenchymal stem cells.
  JOURNAL   J Gene Med. 2010 Oct;12(10):848-58. doi: 10.1002/jgm.1506.
  PUBMED    20963807
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jgm"; 
            journalName: "J Gene Med"; date: "2010-10"; volume: "12"; issue: 
            "10"; pages: "848-58"
COMMENT     Sequence Label: pHCAG-L2EOP
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(61..77)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(61..77)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(74..96)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    85..101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(109..115,116..133,134..139))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    154..175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(292..309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(543..562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcaggcat gcaagcttga gtattctata gtgtcaccta catagctggg cgtaatcatg
       61 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      121 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      181 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      241 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      301 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      361 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      421 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      481 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      541 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      601 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      661 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      721 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      781 cccggta
//
