LOCUS       sequence-81994-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial cloning vector based on pUC21 (Amp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ
  TITLE     Yeast vectors for integration at the HO locus.
  JOURNAL   Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.
  PUBMED    11410682
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001-06-15"; volume: "29(12)"; pages: "E59-9"
COMMENT     Sequence Label: pUC21-NotI
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(29..45)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(29..45)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(224..240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(224..240)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(237..259)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    248..264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    319..340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(457..474)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(708..727)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgactcacta tagggcgaat tggggatcga tccactagtt ctagagcggc cgcataggcc
       61 actagtggat ctgatatcat cgatgaattc gagctcgttt aaacagatct ggcgcgcctt
      121 aattaacccc ggggatccgt cgacctgcag cgtacgaagc ttcagctggc ggccgccacg
      181 gcgatatcgg atctcgaggc ctcggactag tggcgtaatc atggtcatag ctgtttcctg
      241 tgtgaaattg ttatccgctc acaattccac acaacatacg agccgcggaa gcataaagtg
      301 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      361 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      421 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      481 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      541 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      601 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      661 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      721 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      781 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      841 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      901 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      961 cttatcg
//
