LOCUS       sequence-81995-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Kotera I, Iwasaki T, Imamura H, Noji H, Nagai T
  TITLE     Reversible dimerization of Aequorea victoria fluorescent proteins 
            increases the dynamic range of FRET-based indicators.
  JOURNAL   ACS Chem Biol. 2010 Feb 19;5(2):215-22. doi: 10.1021/cb900263z.
  PUBMED    20047338
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Chem Biol"; date: "2010-02-19- 19"; volume: "5"; issue: "2"; 
            pages: "215-22"
COMMENT     Sequence Label: ATeam1.03-nD/nA/pcDNA3
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     447..468
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(571..592)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(832..851)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atcctccccg gtcacattcc cacaaaggcc ccactgaaga tcggagctgt gcggctgaag
       61 aaggacggcc aaaccgagat ggtcgcagtc tcaggcggca ctgttgaagt gcggcctgac
      121 cacgttacca ttaatgctca agccgctgaa acagccgaag gaatcgacaa agagagagca
      181 gaagccgcaa gacagagggc ccaggagcgg ctgaactctc aatccgatga caccgatatt
      241 cgccgggccg agctggcact gcagagggcc ctgaacagac tggacgtggc tgggaaggca
      301 aacgaattca tggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
      361 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
      421 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      481 atcactctcg gcatggacga gctgtacaag ggtggcagcg gtggcatggt gagcaagggc
      541 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      601 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      661 aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      721 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      781 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      841 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      901 ctgaagggca tcg
//
