LOCUS       sequence-81998-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast plasmid for integration of target sequence at HO locus, Kan 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ
  TITLE     Yeast vectors for integration at the HO locus.
  JOURNAL   Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.
  PUBMED    11410682
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001-06-15"; volume: "29(12)"; pages: "E59-9"
COMMENT     Sequence Label: HO-Poly-KanMX4-HO
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(38..381)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 atcgcggcct cgaaacgtga gtcttttcct tacccatggt tgtttatgtt cggatgtgat
       61 gtgagaactg tatcctagca agattttaaa aggaagtata tgaaagaaga acctcagtgg
      121 caaatcctaa ccttttatat ttctctacag gggcgcggcg tggggacaat tcaacgcgtc
      181 tgtgagggga gcgtttccct gctcgcaggt ctgcagcgag gagccgtaat ttttgcttcg
      241 cgccgtgcgg ccatcaaaat gtatggatgc aaatgattat acatggggat gtatgggcta
      301 aatgtacggg cgacagtcac atcatgcccc tgagctgcgc acgtcaagac tgtcaaggag
      361 ggtattctgg gcctccatgt cgctggccgg gtgacccggc ggggacgagg caagctaaac
      421 agatctggcg cgccttaatt aacccgggga tccgtcgacc tgcagcgtac gacgccattt
      481 taagtccaaa ggcacaattt tacgttggaa ctaaacggta aagatcaaat atcaccttta
      541 ttcttttttg gtatgcatcg atttctttgc cagtaagaac tacgtgcaac tctacattag
      601 atcaaaatat aacggatcat aaatgcaaac aacatttgtc caaaagaaac aaaaatgttg
      661 gcgtttgtct cgaacatgac acaatactcc ttgatcgagg gacattgccc gattaactat
      721 gatttatact ttttactaca ggtactcgtt ctccctacta tttcaccagt atacggtcgt
      781 tttatgattc atttattgcc tataatggta aaa
//
