LOCUS       sequence-82006-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  multicopy (YEp) vector with a LYS2 marker and a 2-um origin of 
            replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Eriksson P, Thomas LR, Thorburn A, Stillman DJ
  TITLE     pRS yeast vectors with a LYS2 marker.
  JOURNAL   Biotechniques. 2004 Feb;36(2):212-3.
  PUBMED    14989082
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2004-02"; volume: "36(2)"; pages: "212-3"
COMMENT     Sequence Label: pRS327
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     182..204
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     196..213
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     197..213
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     220..239
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        220..238
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    247..354
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     271..287
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     271..287
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(321..337)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(322..338)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(366..384)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(405..421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(405..421)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(418..440)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    429..445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(453..459,460..477,478..483))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    498..519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(636..653)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
       61 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
      121 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
      181 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc
      241 gaattggagc tccaccgcgg tggcggccgc tctagaacta gtggatcccc cgggctgcag
      301 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg taccagcttt
      361 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
      421 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      481 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      541 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      601 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      661 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      721 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      781 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctc
//
