LOCUS       sequence-82014-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of rat LC3 fused to mRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Kimura S, Noda T, Yoshimori T
  TITLE     Dissection of the autophagosome maturation process by a novel 
            reporter protein, tandem fluorescent-tagged LC3.
  JOURNAL   Autophagy. 2007;3(5):452-60.
  PUBMED    17534139
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2007"; volume: "3(5)"; pages: "452-60"
COMMENT     Sequence Label: pmRFP-LC3
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..43
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(202..405)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(231..251)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        406..709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 caggcgaagg gcagggggcc gcccttggtc accttcagct tggcggtctg ggtgccctcg
       61 taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc
      121 atgcgcacct tgaagcgcat gaactccttg atgacgtcct cggaggaggc catgctagcg
      181 gatctgacgg ttcactaaac cagctctgct tatatagacc tcccaccgta cacgcctacc
      241 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
      301 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
      361 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
      421 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
      481 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
      541 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
      601 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
      661 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
      721 atatgggcta tgaactaatg accccgtaat tgattactat taataactaa tgcatggcgg
      781 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      841 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      901 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      961 tat
//
