LOCUS       sequence-82021-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
  TITLE     Characterizing bacterial gene circuit dynamics with optically 
            programmed gene expression signals.
  JOURNAL   Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
  PUBMED    24608181
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884."
COMMENT     Sequence Label: pJT119b
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(53..155)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
       61 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
      121 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc cagatatcga
      181 cgtcctaagc tcgaggcaaa tggttatagc gttttaaaca gatagtaact gttgttccct
      241 ggtcaactgc actttttagc aagatactgc cactgtgtaa gtcaacacat ttcttggcaa
      301 ccattaaacc gagtcctgtt cccgtaatat ttctgacatt tttgccccga tgaaagggtt
      361 caaaaatttg cttttggtcc tctggcgaaa tgccaatgcc ctggtcggtg acttcaaaaa
      421 taatctgttc cgaatctagg cttagggcaa ttttaatctg ccctcccccg ggagagtatt
      481 taatcgcatt agataacaga ttagataaaa tagaccgcac taacctttca tccaccaatg
      541 ccttcgtatc ttgagcgcta caaataaagt caaaataata ttggtcactg acacttaatt
      601 gaatttcttc gataaattgc tggaacaata atttcaaatc taaccaatta ggattaaatt
      661 ccaatttgcc cgcttcggca cggttaatga ttaaaatatc atccaggagc tgtaccatat
      721 ttttcacgga attttgaata cggtgtaagt tccggctacg cttatcggga tcaagccagg
      781 ccacttcaga attttccagt aattgggcag cagctaaggc cgtactgagg ggagtacgaa
      841 attcatggga agccatggag aaaaaacgag tttttaggcg gcttaattct ttttcccgtt
      901 ctaaagctag gcggacttcc tccgtgcgtc ggcgctcagt gacatccctt tgcacagcaa
      961 tccaatgg
//
