LOCUS       sequence-82033-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Final destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, 
            Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
  TITLE     Repeating pattern of non-RVD variations in DNA-binding modules 
            enhances TALEN activity.
  JOURNAL   Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
  PUBMED    24287550
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-29"; volume: "3:3379. doi"; pages: " 
            10.1038/srep03379"
COMMENT     Sequence Label: ptCMV-153/47-VR-NG
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(56..76)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(56..74)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(95..111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(95..111)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(108..130)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    119..135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(143..149,150..167,168..173))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    188..209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 actatagggc gaattgggta ccgggccccc cctcgaggtc ctccagcttt tgttcccttt
       61 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      121 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      181 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccacc
      241 ggtcgtctcc acccctgagc aggtagtggc tattgcatcc aacggagggg gcagacccgc
      301 actggagtca atcgtggccc agctttcgag gccggacccc gcgctggccg cactcactaa
      361 tgatcatctt gtagcgctgg cctgcctcgg cggacgtcct gccatggatg cagtgaaaaa
      421 gggattgccg cacgcgccgg aattgatcag atcccagcta gtgaaatctg aattggaaga
      481 gaagaaatct gaacttagac ataaattgaa atatgtgcca catgaatata ttgaattgat
      541 tgaaatcgca agaaattcaa ctcaggatag aatccttgaa atgaaggtga tggagttctt
      601 tatgaaggtt tatggttatc gtggtaaaca tttgggtgga tcaaggaaac cagacggagc
      661 aatttatact gtcggatctc ctattgatta cggtgtgatc gttgatacta aggcatattc
      721 aggaggttat aatcttccaa ttggtcaagc agatgaaatg caaagatatg tcgaagagaa
      781 tcaaacaaga aacaagcata tcaaccctaa tgaatggtgg aaa
//
