LOCUS       sequence-82036-e         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the human MAVS CDS containing an N-terminal HA tag and a 
            frameshift mutation inserting TA at bp number 254 of the MAVS CDS . 
            And a GFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Brubaker SW, Gauthier AE, Mills EW, Ingolia NT, Kagan JC
  TITLE     A Bicistronic MAVS Transcript Highlights a Class of Truncated 
            Variants in Antiviral Immunity.
  JOURNAL   Cell. 2014 Feb 13;156(4):800-11. doi: 10.1016/j.cell.2014.01.021.
  PUBMED    24529381
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.01"; journalName: "Cell"; date: "2014-02-13- 
            13"; volume: "156"; issue: "4"; pages: "800-11"
COMMENT     Sequence Label: pMSCV-HA-shift-IRES-GFP(KaganE21)
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    25..577
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    27..593
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(187..206)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     416..433
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ccttgctcac catggttgtg gccatattat catcgtgttt ttcaaaggaa aaccacgtcc
       61 ccgtggttcg gggggcctag acgttttttt aacctcgact aaacacatgt aaagcatgtg
      121 caccgaggcc ccagatcaga tcccatacaa tggggtacct tctgggcatc cttcagcccc
      181 ttgttgaata cgcttgagga gagccatttg actctttcca caactatcca actcacaacg
      241 tggcactggg gttgtgccgc ctttgcaggt gtatcttata cacgtggctt ttggccgcag
      301 aggcacctgt cgccaggtgg ggggttccgc tgcctgcaaa gggtcgctac agacgttgtt
      361 tgtcttcaag aagcttccag aggaactgct tccttcacga cattcaacag accttgcatt
      421 cctttggcga gaggggaaag acccctagga atgctcgtca agaagacagg gccaggtttc
      481 cgggccctca cattgccaaa agacggcaat atggtggaaa ataacatata gacaaacgca
      541 caccggcctt attccaagcg gcttcggcca gtaacgttag gggggggggc ggaattcgat
      601 atcaagctta tcgataccgt cgacgtttaa acgcggccgc cta
//
