LOCUS       sequence-82037-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag SKA1 for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Welburn JP, Grishchuk EL, Backer CB, Wilson-Kubalek EM, Yates JR 
            3rd, Cheeseman IM
  TITLE     The human kinetochore Ska1 complex facilitates microtubule 
            depolymerization-coupled motility.
  JOURNAL   Dev Cell. 2009 Mar;16(3):374-85. doi: 10.1016/j.devcel.2009.01.011.
  PUBMED    19289083
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2009.01"; journalName: "Dev Cell"; date: 
            "2009-03"; volume: "16"; issue: "3"; pages: "374-85"
COMMENT     Sequence Label: pIC291
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..68
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             81..125
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 agtccggagg ggatcggtgg agctccaccg gtggcggccg ctctagagag aatctttatt
       61 ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc cagcacatgg
      121 acagcggagg tggaggtact agtctcgaga tggcctcgtc agatctggaa caattatgct
      181 ctcatgttaa tgaaaagatt ggcaatatta agaaaacctt atcattaaga aactgtggcc
      241 aggaacctac cttgaaaact gtattaaata aaataggaga tgagatcatt gtaataaatg
      301 aacttctaaa taaattggaa ttggaaattc agtatcaaga acaaaccaac aattcactca
      361 aggaactctg tgaatctctt gaagaagatt acaaagacat agaacatctt aaagaaaacg
      421 ttccttccca tttgcctcaa gtaacagtaa cccagagctg tgttaaggga tcagatcttg
      481 atcctgaaga accaatcaaa gttgaagaac ctgaacccgt aaagaagcct cccaaagagc
      541 aaagaagtat taaggaaatg ccatttataa cttgtgatga gttcaatggt gttccttcgt
      601 acatgaaatc ccgcttaacc tataatcaaa ttaatgatgt tattaaagaa atcaacaagg
      661 cagtaattag taaatataaa atcctacatc agccaaaaaa gtctatgaat tctgtgacca
      721 gaaatctcta tcacagattt attgatgaag aaacgaagga taccaaaggt cgtt
//
