LOCUS       sequence-82040-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial cloning vector based on pUK21 (Kan).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ
  TITLE     Yeast vectors for integration at the HO locus.
  JOURNAL   Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.
  PUBMED    11410682
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001-06-15"; volume: "29(12)"; pages: "E59-9"
COMMENT     Sequence Label: pUK21-deltaBB
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(29..45)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(29..45)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(101..117)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(101..117)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(114..136)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    125..141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    196..217
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(334..351)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(585..604)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgactcacta tagggcgaat tggggatcga tccactagtt ctagagcggc cgccacggcg
       61 atatcggatc tcgaggcctc ggactagtgg cgtaatcatg gtcatagctg tttcctgtgt
      121 gaaattgtta tccgctcaca attccacaca acatacgagc cgcggaagca taaagtgtaa
      181 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      241 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      301 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      361 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      421 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      481 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      541 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      601 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      661 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      721 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      781 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      841 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg ta
//
