LOCUS       sequence-82041-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag SKA2 for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Welburn JP, Grishchuk EL, Backer CB, Wilson-Kubalek EM, Yates JR 
            3rd, Cheeseman IM
  TITLE     The human kinetochore Ska1 complex facilitates microtubule 
            depolymerization-coupled motility.
  JOURNAL   Dev Cell. 2009 Mar;16(3):374-85. doi: 10.1016/j.devcel.2009.01.011.
  PUBMED    19289083
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2009.01"; journalName: "Dev Cell"; date: 
            "2009-03"; volume: "16"; issue: "3"; pages: "374-85"
COMMENT     Sequence Label: pIC292
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..68
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             81..125
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(691..711)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 agtccggagg ggatcggtgg agctccaccg gtggcggccg ctctagagag aatctttatt
       61 ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc cagcacatgg
      121 acagcggagg tggaggtact agtctcgaga tggaggcgga ggtcgataag ctggaactga
      181 tgttccagaa agctgagtct gatctggatt acattcaata caggctggaa tatgaaatca
      241 agactaatca tcctgattca gcaagtgaga aaaatccagt tacactctta aaggaattgt
      301 cagtgataaa gtctcgatat caaactttgt atgcccgctt taaaccagtt gctgttgagc
      361 agaaagagag taagagccgc atttgtgcta ctgtgaaaaa gactatgaat atgatacaaa
      421 aactacagaa gcaaacagac ctggagctgt caccactgac taaagaagag aaaactgcgg
      481 cagagcaatt caaatttcac atgccagatt tatgagaatt ctgcagtcga cggtaccgcg
      541 ggcccgggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
      601 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      661 gcaattcgcc agcacagtgg tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt
      721 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      781 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
      841 agtcagcaac catagtcccg cccctaactc cgccc
//
