LOCUS       sequence-82043-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag SKA3 for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Welburn JP, Grishchuk EL, Backer CB, Wilson-Kubalek EM, Yates JR 
            3rd, Cheeseman IM
  TITLE     The human kinetochore Ska1 complex facilitates microtubule 
            depolymerization-coupled motility.
  JOURNAL   Dev Cell. 2009 Mar;16(3):374-85. doi: 10.1016/j.devcel.2009.01.011.
  PUBMED    19289083
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2009.01"; journalName: "Dev Cell"; date: 
            "2009-03"; volume: "16"; issue: "3"; pages: "374-85"
COMMENT     Sequence Label: pIC306
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..68
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             81..125
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 agtccggagg ggatcggtgg agctccaccg gtggcggccg ctctagagag aatctttatt
       61 ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc cagcacatgg
      121 acagcggagg tggaggtact agtctcgaga tggaccctat ccggagcttc tgcgggaagc
      181 tgcggtctct ggccagcacg ctggactgcg agacggcccg gctgcagcga gcgctggacg
      241 gagaggaaag cgactttgaa gattatccaa tgagaatttt atatgacctt cattcagaag
      301 ttcagactct aaaggatgat attaatattc ttcttgataa agcaagattg gaaaatcaag
      361 aaggcattga tttcataaag gcaacaaaag tactaatgga aaaaaattca atggatatta
      421 tgaaaataag agagtatttc cagaagtatg gatatagtcc acgtgtcaag aaaaattcag
      481 tacacgagca agaagccatt aactctgacc cagagttgtc taattgtgaa aattttcaga
      541 agactgatgt gaaagatgat ctgtctgatc ctcctgttgc aagcagttgt atttctgaga
      601 agtctccacg tagtccacaa ctttcagatt ttggacttga gcggtacatc gtatcccaag
      661 ttctaccaaa ccctccacag gcagtgaaca actataagga agagcccgta attgtaaccc
      721 cacctaccaa acaatcacta gtaaaagtac taaaaactcc aaaatgtgca ctaaaaatgg
      781 atgattttga gtgtgtaact cctaaattag aacactttgg tatctctgaa tatactatgt
      841 gtttaaatga agattacaca atggg
//
