LOCUS       sequence-82048-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Kawaoka S, Hayashi N, Suzuki Y, Abe H, Sugano S, Tomari Y, Shimada 
            T, Katsuma S
  TITLE     The Bombyx ovary-derived cell line endogenously expresses 
            PIWI/PIWI-interacting RNA complexes.
  JOURNAL   RNA. 2009 Jul;15(7):1258-64. doi: 10.1261/rna.1452209. Epub 2009 May
            21.
  PUBMED    19460866
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1452209"; journalName: "RNA"; date: "2009-07"; volume: 
            "15"; issue: "7"; pages: "1258-64"
COMMENT     Sequence Label: pIZ-Flag6His-Siwi
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(561..578)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(579..602)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcctttcgga ctccagtgct gtcttcttct ggagaaatat cgacgtgata ttgatacagg
       61 ccccatttgg gagttgtttc aacagtaaaa tagttggcaa gcagatccag gggagtaccc
      121 gaagtacctt tcttagatgt tacagcttct ggacgtgtgc gcaagatgga aatagtttca
      181 ggaaggactc ttccaccccc tcgtcgagag ccacggccaa ctacggatga tgcatcacca
      241 cctccagatg aatggggtcc cagttctaat ttttgcatgc gttgttgcac atcgatatct
      301 cctggatgtt catgtgtagt gggagtaaca cggtgtgagg cgcgacccgc ttgtgctgtt
      361 ggtgtcggga caccagctct tactggagga acactggatt gtggtcccca gccagatgga
      421 ggttgaggtc taggaccagg tggcatagat tgttgtgatg ggcctgcttg ttcccctgga
      481 cgtcgtggtg ctggtccgcc tccatcacca ccacgaccag cgcgtcctcg agctcgtcct
      541 ctacctctcg gttctgacat atgatgatga tgatgatgct tatcgtcgtc atccttgtaa
      601 tccatggatc cgagctcggt accaagcttt aaattcgaac agatgctgtt caactgtgtt
      661 taccagatcg ttgcgggctg tatttatagg cgcgataagc gggacgggcg cctcgtgtcc
      721 ggtcacgcgc atgagataac gcgcggctga tatggaggcg cgtcctgttc cgataaggag
      781 tt
//
