LOCUS       sequence-82049-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NRPS module of ClbB containing CAT domains with N-terminal 6His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Brotherton CA, Balskus EP
  TITLE     A prodrug resistance mechanism is involved in colibactin 
            biosynthesis and cytotoxicity.
  JOURNAL   J Am Chem Soc. 2013 Mar 6;135(9):3359-62. doi: 10.1021/ja312154m. 
            Epub 2013 Feb 20.
  PUBMED    23406518
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja312154m";
            journalName: "J Am Chem Soc"; date: "2013-03-6- 6"; volume: "135"; 
            issue: "9"; pages: "3359-62"
COMMENT     Sequence Label: pet28a-ClbB-NRPS-NHis
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tcacgttgga attgcatcct
       61 ccggcgatac ggcagtatcg agaaaatgtg ctaacgccgt gagcgttggg tacttaaaga
      121 gatcggtaat atcaatctcc aagccaagtt gccgcttaat atccccttta accctgatgg
      181 tatccaatga ggtgccaccg agggcgaaaa aggaggcatt gctatcaatg tcttgtctat
      241 caagtacgcg ttgccagata gcatgcagcg catgttccat gtcactatgc acctctgata
      301 tcgtggtgga cacggcgttg cactgagttg cgggcaggag ataccgcaac tgcgccaagt
      361 cggccttgcc actatggctt tttgcaatct ccgtcaccac caccaaggat tccggaatag
      421 cgaatggcgg caatctgtca gccaattgcc ctcgaacttc cacctcatca tagctatcat
      481 tgacgcagac cactgccgca atatgcagcg tttcacgtac gcggtaagga accactaccg
      541 cctcgataat gccggagcac tccagcaggc gctgctcgat ctctccggtt tcaattcgtt
      601 gtccattgat cttaatttga ccgtctttac gcccaaaaca gtcaacacag ccatcatcca
      661 gcaaccgggc ataatcgcca gttttgtaca tcataccgcc gggaataaaa ggatcaggga
      721 caaaaaccgt cgcacttagc gccgggttgt tctgatagcc gatggcgacc cccactccac
      781 ccgccactag ttcaccggtg gcaccgatag gcaa
//
