LOCUS       sequence-82050-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human SPOP (aa 28-337) fused to His-MBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, 
            Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, 
            White KP, Schulman BA
  TITLE     Structures of SPOP-substrate complexes: insights into molecular 
            architectures of BTB-Cul3 ubiquitin ligases.
  JOURNAL   Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022.
  PUBMED    19818708
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.09"; journalName: "Mol Cell"; date: 
            "2009-10-9- 9"; volume: "36"; issue: "1"; pages: "39-50"
COMMENT     Sequence Label: His-MBP human SPOP (aa 28-337)
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..50)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    159..183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(183..202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(184..202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt accagactcg
       61 agggtaccga cgtcagcgat cgcgtggccg gccgatatcc aattgagatc tgccatatgt
      121 atatctcctt cttatactta actaatatac taagatgggg aattgttatc cgctcacaat
      181 tcccctatag tgagtcgtat taatttcgat tatgcggccg tgtacaatac gattactttc
      241 tgttcgactt aagcattatg cggccgcaag cttgtcgact tacccagagg tctccaagac
      301 atccgaagca tgatagttga taaaatccac tgcctgagtt ttcaactgat ctgcactgtg
      361 gaggtcggcc aggatgagaa tttctgcagc gttctccacg gacaggttac tgcagagggc
      421 atcctcacac atgaccttta agcgctccag ggcatacttg tcagcagctg ccagcaaatc
      481 atcagccatt ttgtcgaggt ttggagcctt ccccgtgtaa atgaagcaca tcatttcctt
      541 aaaaacttca ggctccacat cattgatttc aactcgattc tttttgctct cctccatttc
      601 atgttcaaac atggcactaa aaaccggaga acgagctgct aagatagcct tgtgagcctg
      661 gaattcctgg ccggcaacac acaagcagca gtctgtgaac cgggaattct cccacagtcc
      721 tcctaactca tctgccagcc ggcactcagg aacctttacc atgttcatgg tattctggcc
      781 agaaa
//
