LOCUS       sequence-82052-e        3075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KIlac with a codon optimized lacZ using preferred codon at every 
            position.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Eames M, Kortemme T
  TITLE     Cost-benefit tradeoffs in engineered lac operons.
  JOURNAL   Science. 2012 May 18;336(6083):911-5. doi: 10.1126/science.1219083.
  PUBMED    22605776
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2012-05-18- 18"; volume: "336"; 
            issue: "6083"; pages: "911-5"
COMMENT     Sequence Label: OpLac1
FEATURES             Location/Qualifiers
     source          1..3075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..3075
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
ORIGIN
        1 atgaccatga ttaccgatag cctggccgtg gtgctgcagc gtcgtgattg ggaaaatccg
       61 ggcgtgacgc agctgaatcg tctggccgcg catccgccgt ttgcgagctg gcgtaacagc
      121 gaagaagcgc gtaccgatcg tccgagccag cagctgcgta gcctgaacgg cgaatggcgt
      181 tttgcgtggt ttccggcacc ggaggcggtt ccggaaagct ggctggaatg cgatctgccg
      241 gaagcggata ccgtggtggt gccgagcaac tggcagatgc atggctatga tgcgccgatt
      301 tataccaacg tgacctatcc gattaccgtg aatccgccgt tcgtgccgac cgaaaatccg
      361 accggctgct atagcctgac ctttaacgtg gatgaatctt ggctgcagga aggccagacc
      421 cgtattattt ttgatggcgt gaacagcgcg tttcatctgt ggtgcaatgg ccgctgggtg
      481 ggctatggcc aggatagccg tctgccgagc gaatttgatc tgagcgcgtt tctgcgtgcg
      541 ggcgaaaacc gtctggccgt gatggtgctg cgttggtctg atggcagcta tctggaagat
      601 caggatatgt ggcgtatgag cggcattttt cgtgatgtga gcctgctgca taaaccgacc
      661 acccagatta gcgattttca tgtggcgacc cgttttaacg atgattttag ccgtgcggtg
      721 ctggaagcgg aagtgcagat gtgcggcgaa ctgcgtgatt atctgcgtgt gaccgtgagc
      781 ctgtggcagg gcgaaacgca ggttgcgtct ggcaccgcgc cgtttggcgg cgaaattatt
      841 gatgaacgtg gcggctatgc ggatcgtgtg accctgcgtc tgaacgtgga aaatccgaaa
      901 ctgtggagcg cggaaattcc gaacctgtat cgtgcggtgg tggaactgca taccgcggat
      961 ggcaccctga ttgaagcgga agcgtgtgat gtgggctttc gtgaagtgcg tattgaaaac
     1021 ggcctgctgc tgctgaacgg caaaccgctg ctgattcgtg gcgtgaaccg tcatgaacat
     1081 catccgctgc atggccaggt gatggatgaa cagaccatgg tgcaggatat tctgctgatg
     1141 aaacagaaca actttaacgc ggtgcgttgc agccattatc cgaaccatcc gctgtggtat
     1201 accctgtgcg atcgttatgg cctgtatgtg gtggatgaag cgaacattga aacccatggc
     1261 atggtgccga tgaaccgtct gaccgatgat ccgcgttggc tgccggccat gagcgaacgt
     1321 gtgacccgta tggtgcagcg tgatcgtaac catccgagcg tgattatttg gagcctgggc
     1381 aacgaaagcg gccatggcgc gaaccatgat gcgctgtatc gttggattaa aagcgtggat
     1441 ccgagccgtc cggtgcagta tgaaggcggc ggtgcggata ccaccgcgac cgatattatt
     1501 tgcccgatgt atgcgcgtgt ggatgaagat cagccgtttc cggcggtgcc gaaatggtct
     1561 attaaaaaat ggctgtctct gccgggcgaa acgcgtccgc tgattctgtg cgaatatgcg
     1621 catgccatgg gcaacagcct gggcggcttt gcgaaatatt ggcaggcgtt tcgtcagtat
     1681 ccgcgtctgc agggcggctt tgtgtgggat tgggtggatc agagcctgat caaatatgat
     1741 gaaaacggca atccgtggag cgcgtatggc ggcgattttg gcgatacccc gaacgatcgt
     1801 cagttttgca tgaacggcct ggtgtttgcg gatcgtaccc cgcatccggc gctgaccgaa
     1861 gcgaaacatc agcagcagtt ttttcagttt cgtctgagcg gccagaccat tgaagtgacc
     1921 agcgaatacc tgtttcgtca tagcgataac gaactgctgc attggatggt ggcgctggat
     1981 ggtaaaccgt tagcatctgg cgaagtgccg ctggatgttg cgccgcaggg caaacagctg
     2041 attgaactgc cggaactgcc gcagccggaa agcgcgggtc agctgtggct gaccgtgcgt
     2101 gtggtgcagc cgaatgcgac cgcgtggagc gaagcgggcc atattagcgc gtggcagcag
     2161 tggcgtctgg ccgaaaacct gagcgttacc ctgccggcag cgagccatgc gattccgcat
     2221 ctgaccacct ctgaaatgga tttttgcatc gaactgggca acaaacgttg gcagtttaac
     2281 cgtcagagcg gctttctgag ccagatgtgg attggcgata aaaaacagct gctgaccccg
     2341 ctgcgtgatc agtttacccg tgcgccgctg gataacgata ttggcgtgag cgaagcgacc
     2401 cgtattgatc cgaacgcgtg ggtggaacgt tggaaagcgg ccggtcatta tcaggcggaa
     2461 gcggcgctgc tgcagtgtac cgcggatacc ctggccgatg cggtgctgat taccaccgcc
     2521 catgcgtggc agcatcaggg caaaacgctg tttattagcc gtaaaaccta tcgtattgat
     2581 ggcagcggcc agatggcgat taccgtggat gtggaagtgg cgagcgatac cccgcacccg
     2641 gcacgtattg gcctgaactg ccagctggcc caggtggcgg aacgtgtgaa ctggctgggc
     2701 ctgggcccgc aggaaaacta tccggatcgt ctgaccgcgg cgtgctttga tcgttgggat
     2761 ctgccgctgt ctgatatgta taccccgtat gtgtttccga gcgaaaacgg tctgcgttgc
     2821 ggcacccgtg aactgaacta tggcccgcat cagtggcgtg gcgattttca gtttaacatc
     2881 agccgttata gccagcagca gctgatggaa accagccatc gtcatctgct gcatgcggaa
     2941 gaaggcacct ggctgaacat tgatggcttt catatgggca ttggcggtga tgattcttgg
     3001 agcccgagcg tgagcgcgga atttcagctg tctgcgggcc gttatcatta tcagctggtg
     3061 tggtgccaga aataa
//
