LOCUS       sequence-82057-e        3075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KIlac with a codon optimized lacZ using codons for a subset of amino
            acids read by transfer RNAs that are the most highly charged during 
            amino acid starvation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Eames M, Kortemme T
  TITLE     Cost-benefit tradeoffs in engineered lac operons.
  JOURNAL   Science. 2012 May 18;336(6083):911-5. doi: 10.1126/science.1219083.
  PUBMED    22605776
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2012-05-18- 18"; volume: "336"; 
            issue: "6083"; pages: "911-5"
COMMENT     Sequence Label: OpLac2
FEATURES             Location/Qualifiers
     source          1..3075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..3075
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
ORIGIN
        1 atgaccatga tcaccgattc tctggctgtg gtattgcagc gtcgcgactg ggagaaccct
       61 ggtgtcaccc aactgaatcg tttggcagcg cacccaccgt tcgcatcttg gcgtaactcg
      121 gaggaggctc gtaccgaccg tccgtcccag caactgcgta gcctgaatgg tgagtggcgt
      181 ttcgcatggt ttccagcacc ggaagccgtt ccggaatcct ggctggagtg tgatctgcca
      241 gaggctgaca ctgttgttgt tccgtccaac tggcagatgc acggttatga cgcaccgatc
      301 tacacgaacg tgacgtaccc gatcaccgtg aatccaccgt ttgtgccgac cgagaatcct
      361 accggttgct atagcctgac cttcaatgtc gacgagtctt ggttgcagga gggtcagacg
      421 cgtatcatct ttgatggtgt gaactctgca ttccatctgt ggtgcaatgg tcgttgggtg
      481 ggttatggcc aagactctcg cctgccttcg gagttcgatc tgtcggcttt cttgcgtgca
      541 ggtgaaaacc gtctggctgt catggtactg cgctggtcgg atggttcgta cttggaagac
      601 caagatatgt ggcgtatgtc gggtatcttt cgcgacgtta gcttgctgca caaaccgact
      661 acccaaatca gcgatttcca tgtcgcgacc cgtttcaacg atgactttag ccgtgcggtt
      721 ctggaagcgg aagtgcaaat gtgtggcgaa ttgcgtgatt acttgcgtgt gacggttagc
      781 ctgtggcagg gtgagacgca agtggcgtcc ggtactgcac ctttcggtgg cgagatcatc
      841 gacgaacgtg gtggttacgc tgatcgcgtg accctgcgct tgaatgtgga gaatccgaaa
      901 ctgtggagcg ctgagattcc gaacttgtac cgtgcagttg tggagctgca caccgcagac
      961 ggcaccttga ttgaggctga agcctgcgat gtgggttttc gcgaagtccg tatcgagaat
     1021 ggtctgttgc tgttgaacgg taaaccgctg ctgattcgtg gcgtgaatcg tcacgagcac
     1081 cacccactgc atggtcaagt gatggatgaa caaacgatgg tgcaagacat tctgctgatg
     1141 aagcagaaca acttcaacgc agttcgctgt agccattacc cgaatcaccc tctgtggtac
     1201 actctgtgcg acagatacgg tctgtacgtt gtcgacgagg cgaacattga aacccatggt
     1261 atggtcccga tgaatcgcct gacggacgac ccacgttggt tgccagctat gagcgaacgc
     1321 gttacccgta tggttcaacg cgaccgtaat catccgagcg tgatcatttg gtccctgggc
     1381 aacgagtcgg gtcacggtgc aaatcacgac gcgttgtatc gttggatcaa aagcgtcgat
     1441 ccatcccgtc cggtgcagta tgagggtggt ggtgcggaca ccaccgctac ggacatcatc
     1501 tgtccgatgt atgcacgcgt cgacgaagat caaccgtttc ctgcggttcc gaaatggagc
     1561 atcaagaaat ggttgtcgct gcctggtgaa acccgtccac tgatcttgtg tgagtacgca
     1621 catgctatgg gcaattctct gggtggcttc gcaaagtact ggcaggcgtt tcgccagtat
     1681 ccacgcttgc aaggtggttt cgtttgggac tgggtggacc aatctttgat caagtacgac
     1741 gaaaacggca atccatggtc ggcatacggt ggcgatttcg gtgatacccc aaacgaccgt
     1801 cagttctgca tgaacggctt ggtattcgct gaccgtaccc ctcatcctgc gttgacggaa
     1861 gcgaaacatc aacaacagtt cttccaattc cgtctgtcgg gtcagaccat tgaagtgacc
     1921 tccgagtacc tgttccgtca cagcgacaac gagttgctgc actggatggt cgcgttggac
     1981 ggcaagccgt tggcgtcggg tgaggtgccg ttggacgttg caccgcaagg caagcaactg
     2041 atcgaactgc ctgaactgcc acaaccggaa agcgctggcc aactgtggct gaccgttcgt
     2101 gtggtgcagc cgaatgcaac tgcgtggagc gaggctggtc acatttctgc ctggcagcag
     2161 tggcgtctgg cagagaactt gtccgttact ctgcctgctg cgtcgcatgc gattccgcat
     2221 ttgaccacct cggagatgga cttctgtatt gagctgggca acaagcgctg gcaattcaat
     2281 cgccagagcg gttttctgtc gcaaatgtgg attggcgaca agaagcaact gctgacccct
     2341 ttgcgtgacc aattcaccag agcaccgctg gacaatgaca ttggtgttag cgaggcgacc
     2401 agaattgacc caaatgcgtg ggtggaacgt tggaaggctg ctggccacta tcaagcggaa
     2461 gctgccctgc tgcaatgtac cgcagatacg ctggctgacg ctgtgctgat caccactgcc
     2521 cacgcgtggc agcatcaagg caagaccctg ttcatctcgc gtaagaccta tcgtattgac
     2581 ggctcgggtc aaatggccat taccgttgac gtggaagtgg ccagcgatac ccctcaccca
     2641 gcacgcatcg gtctgaattg tcaattggct caggttgcgg aacgtgtgaa ctggttgggt
     2701 ctgggtccgc aagaaaacta tccggaccgc ttgaccgctg catgcttcga ccgttgggac
     2761 ctgccactgt ctgacatgta cacgccatac gttttcccga gcgagaatgg tctgcgctgc
     2821 ggtactcgcg aactgaacta cggtccacat caatggagag gcgacttcca gttcaacatc
     2881 tccagataca gccagcaaca gttgatggag acgagccatc gccatctgct gcatgctgag
     2941 gaaggtacct ggttgaacat tgacggcttc catatgggca ttggtggtga cgatagctgg
     3001 agcccatcgg tgtctgccga gttccaactg tctgctggtc gctaccacta tcaattggtt
     3061 tggtgccaga agtaa
//
