LOCUS       sequence-8206-ex       10899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Mena MA, Treynor TP, Mayo SL, Daugherty PS
  TITLE     Blue fluorescent proteins with enhanced brightness and 
            photostability from a structurally targeted library.
  JOURNAL   Nat Biotechnol. 2006 Dec . 24(12):1569-71.
  PUBMED    17115054
REFERENCE   2  (bases 1 to 10899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2006 Dec . 24(12):1569-71."
COMMENT     Sequence Label: pCEP4Azurite-Mamm
FEATURES             Location/Qualifiers
     source          1..10899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     538..558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     584..608
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      648..657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(960..979)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    1385..1519
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1409..1428)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1463..1482
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1662..1684)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2242..4031
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4333..6258)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6693..6711)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6779..6883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6884..6952,6953..7744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7102..7121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7915..8503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8404..8423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8934..9079
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9122..10159
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(10157..10176)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10201..10249
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccgcggg cccgggatcc accggtcgcc accatggtga
      661 gcaaaggcga agagctgttc accggcgtgg tgcccatcct ggtggagctg gacggcgacg
      721 tgaacggcca caagttcagc gtgagcggcg agggcgaggg cgacgccacc tacggcaagc
      781 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctggtga
      841 ccaccctgag ccacggcgtg cagtgcttca gccggtaccc cgaccacatg aagcagcacg
      901 acttcttcaa gagcgccatg cccgagggct acgtgcagga gcggaccatc ttcttcaagg
      961 acgacggcaa ctacaagacc cgggccgagg tgaagttcga gggcgacacc ctggtgaacc
     1021 ggatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggc cacaagctgg
     1081 agtacaactt caacagccac aacatctaca tcatggccga caagcagaag aacggcatca
     1141 aggtgaactt caagatccgg cacaacatcg aggacggcag cgtgcagctg gccgaccact
     1201 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1261 gcacccagag cgccctgagc aaggacccca acgagaagcg ggaccacatg gtgctgctgg
     1321 agttccggac cgccgccggc atcacccacg gcatggacga gctctataag taaagcggcc
     1381 aacggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     1441 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     1501 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     1561 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     1621 tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     1681 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcaggcg
     1741 tcagcgggtg ttggcgggtg tcggggcgca gccatgaggt cgactctaga ggatcgatgc
     1801 cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg
     1861 catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac
     1921 acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga
     1981 tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga
     2041 ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg
     2101 gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag
     2161 aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat
     2221 aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta
     2281 tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat
     2341 tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg
     2401 ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca
     2461 gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt
     2521 ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt
     2581 gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg
     2641 gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag
     2701 tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc
     2761 cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag
     2821 tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc
     2881 atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact
     2941 ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat
     3001 aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc
     3061 cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg
     3121 taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg
     3181 ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga
     3241 ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc
     3301 acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat
     3361 atctggatag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     3421 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     3481 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     3541 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag
     3601 attagggtag tatatgctat cctaatttat atctgggtag catatactac ccaaatatct
     3661 ggatagcata tgctatccta atctatatct gggtagcata tgctatccta atctatatct
     3721 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     3781 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     3841 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
     3901 gggtagcata tgctatcctc atgcatatac agtcagcata tgatacccag tagtagagtg
     3961 ggagtgctat cctttgcata tgccgccacc tcccaagggg gcgtgaattt tcgctgcttg
     4021 tccttttcct gctggttgct cccattctta ggtgaattta aggaggccag gctaaagccg
     4081 tcgcatgtct gattgctcac caggtaaatg tcgctaatgt tttccaacgc gagaaggtgt
     4141 tgagcgcgga gctgagtgac gtgacaacat gggtatgccc aattgcccca tgttgggagg
     4201 acgaaaatgg tgacaagaca gatggccaga aatacaccaa cagcacgcat gatgtctact
     4261 ggggatttat tctttagtgc gggggaatac acggctttta atacgattga gggcgtctcc
     4321 taacaagtta catcactcct gcccttcctc accctcatct ccatcacctc cttcatctcc
     4381 gtcatctccg tcatcaccct ccgcggcagc cccttccacc ataggtggaa accagggagg
     4441 caaatctact ccatcgtcaa agctgcacac agtcaccctg atattgcagg taggagcggg
     4501 ctttgtcata acaaggtcct taatcgcatc cttcaaaacc tcagcaaata tatgagtttg
     4561 taaaaagacc atgaaataac agacaatgga ctcccttagc gggccaggtt gtgggccggg
     4621 tccaggggcc attccaaagg ggagacgact caatggtgta agacgacatt gtggaatagc
     4681 aagggcagtt cctcgcctta ggttgtaaag ggaggtctta ctacctccat atacgaacac
     4741 accggcgacc caagttcctt cgtcggtagt cctttctacg tgactcctag ccaggagagc
     4801 tcttaaacct tctgcaatgt tctcaaattt cgggttggaa cctccttgac cacgatgctt
     4861 tccaaaccac cctccttttt tgcgcctgcc tccatcaccc tgaccccggg gtccagtgct
     4921 tgggccttct cctgggtcat ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg
     4981 ctcaccatct gggccacctt cttggtggta ttcaaaataa tcggcttccc ctacagggtg
     5041 gaaaaatggc cttctacctg gagggggcct gcgcggtgga gacccggatg atgatgactg
     5101 actactggga ctcctgggcc tcttttctcc acgtccacga cctctccccc tggctctttc
     5161 acgacttccc cccctggctc tttcacgtcc tctaccccgg cggcctccac tacctcctcg
     5221 accccggcct ccactacctc ctcgaccccg gcctccactg cctcctcgac cccggcctcc
     5281 acctcctgct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc
     5341 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc
     5401 ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     5461 tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     5521 tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc
     5581 ccctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     5641 ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctcc
     5701 tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc
     5761 tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     5821 tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc
     5881 tgctcctgcc cctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     5941 tcctgctcct gcccctcctg ctcctgcccc tcccgctcct gctcctgctc ctgttccacc
     6001 gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga caccatctct
     6061 atgtcttggc cctgatcctg agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg
     6121 tcctcttccc cgtcctcgtc catggttatc accccctctt ctttgaggtc cactgccgcc
     6181 ggagccttct ggtccagatg tgtctccctt ctctcctagg ccatttccag gtcctgtacc
     6241 tggcccctcg tcagacatga ttcacactaa aagagatcaa tagacatctt tattagacga
     6301 cgctcagtga atacagggag tgcagactcc tgccccctcc aacagccccc ccaccctcat
     6361 ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc tccgaaccat
     6421 cctcgtcctc atcaccaatt actcgcagcc cggaaaactc ccgctgaaca tcctcaagat
     6481 ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc cccctttttg ctggacggta
     6541 gggatgggga ttctcgggac ccctcctctt cctcttcaag gtcaccagac agagatgcta
     6601 ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc gatgataagc
     6661 tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     6721 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     6781 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6841 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6901 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6961 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7021 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7081 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     7141 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7201 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7261 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7321 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7381 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
     7441 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7501 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7561 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7621 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7681 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7741 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7801 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7861 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7921 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7981 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8041 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     8101 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8161 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8221 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8281 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8341 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8401 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8461 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8521 ctttttacgg ttcctggcct tttgctggcc ttgaagctgt ccctgatggt cgtcatctac
     8581 ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat
     8641 cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc
     8701 gtcggccccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc
     8761 tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt
     8821 ggagtggtga atccgttagc gaggtgccgc cctgcttcat ccccgtggcc cgttgctcgc
     8881 gtttgctggc ggtgtccccg gaagaaatat atttgcatgt ctttagttct atgatgacac
     8941 aaaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag tcggggcggc
     9001 gcggtccgag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac accgagcgac
     9061 cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca gatcccgggg ggcaatgaga
     9121 tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     9181 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     9241 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     9301 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     9361 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     9421 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     9481 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     9541 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     9601 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     9661 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     9721 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     9781 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     9841 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     9901 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     9961 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
    10021 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
    10081 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cggatcggga
    10141 gatgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
    10201 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
    10261 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
    10321 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
    10381 gcagccaacg tcggggcggc aggccctgcc atagccactg gccccgtggg ttagggacgg
    10441 ggtcccccat ggggaatggt ttatggttcg tgggggttat tattttgggc gttgcgtggg
    10501 gtcaggtcca cgactggact gagcagacag acccatggtt tttggatggc ctgggcatgg
    10561 accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg gctgccaaac acccccgacc
    10621 cccaaaaacc accgcgcgga tttctggcgt gccaagctag tcgaccaatt ctcatgtttg
    10681 acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt
    10741 aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
    10801 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
    10861 tgtacattta tattggctca tgtccaatat gaccgccat
//
