LOCUS       sequence-82062-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encode hSpCas9 for in vitro transcription with Sp6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Ansai S, Kinoshita M
  TITLE     Targeted mutagenesis using CRISPR/Cas system in medaka.
  JOURNAL   Biol Open. 2014 Apr 11;3(5):362-71. doi: 10.1242/bio.20148177.
  PUBMED    24728957
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/bio"; 
            journalName: "Biol Open"; date: "2014-04-11- 11"; volume: "3"; 
            issue: "5"; pages: "362-71"
COMMENT     Sequence Label: pCS2+hSpCas9
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..72)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 acgactcact atagttctag attacttttt cttttttgcc tggccggcct ttttcgtggc
       61 cgccggcctt ttgtcgcctc ccagctgaga caggtcgatc cgtgtctcgt acaggccggt
      121 gatgctctgg tggatcaggg tggcgtccag cacctctttg gtgctggtgt acctcttccg
      181 gtcgatggtg gtgtcaaagt acttgaaggc ggcaggggct cccagattgg tcagggtaaa
      241 caggtggatg atattctcgg cctgctctct gatgggctta tcccggtgct tgttgtaggc
      301 ggacagcact ttgtccagat tagcgtcggc caggatcact ctcttggaga actcgctgat
      361 ctgctcgatg atctcgtcca ggtagtgctt gtgctgttcc acaaacagct gtttctgctc
      421 attatcctcg ggggagccct tcagcttctc atagtggctg gccaggtaca ggaagttcac
      481 atatttggag ggcagggcca gttcgtttcc cttctgcagt tcgccggcag aggccagcat
      541 tctcttccgg ccgttttcca gctcgaacag ggagtactta ggcagcttga tgatcaggtc
      601 ctttttcact tctttgtagc ccttggcttc cagaaagtcg atgggattct tctcgaagct
      661 gcttctttcc atgatggtga tccccagcag ctctttcaca ctcttcagtt tcttggactt
      721 gcccttttcc actttggcca ccaccagcac agaataggcc acggtggggc tgtcgaagcc
      781 gccgtacttc ttagggtccc agtccttctt tctggcgatc agcttatcgc tgttcctctt
      841 gggcaggata gactctttgc tgaagccgcc tgtctgcacc tcggtctttt t
//
