LOCUS       sequence-82063-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encode hSpCas9 for in vitro transcription with Sp6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Ansai S, Kinoshita M
  TITLE     Targeted mutagenesis using CRISPR/Cas system in medaka.
  JOURNAL   Biol Open. 2014 Apr 11;3(5):362-71. doi: 10.1242/bio.20148177.
  PUBMED    24728957
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/bio"; 
            journalName: "Biol Open"; date: "2014-04-11- 11"; volume: "3"; 
            issue: "5"; pages: "362-71"
COMMENT     Sequence Label: pCS2+hSpCas9
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgcctctctc ggccttggtc agattgtcga actttctctg ggtaatcagc ttggcgttca
       61 gcagctgccg ccagtagttc ttcatcttct tcacgacctc ttcggagggc acgttgtcgc
      121 tcttgccccg gttcttgtcg cttctggtca gcaccttgtt gtcgatggag tcgtccttca
      181 gaaagctctg aggcacgata tggtccacat cgtagtcgga cagccggttg atgtccagtt
      241 cctggtccac gtacatatcc cgcccattct gcaggtagta caggtacagc ttctcgttct
      301 gcagctgggt gttttccacg gggtgttctt tcaggatctg gctgcccagc tctttgatgc
      361 cctcttcgat ccgcttcatt ctctcgcggc tgttcttctg tcccttctgg gtggtctggt
      421 tctctctggc catttcgatc acgatgttct cgggcttgtg ccggcccatc actttcacga
      481 gctcgtccac caccttcact gtctgcagga tgcccttctt aatggcgggg ctgccggcca
      541 gattggcaat gtgctcgtgc aggctatcgc cctggccgga cacctgggct ttctggatgt
      601 cctctttaaa ggtcaggctg tcgtcgtgga tcagctgcat gaagtttctg ttggcgaagc
      661 cgtcggactt caggaaatcc aggattgtct tgccggactg cttgtcccgg atgccgttga
      721 tcagcttccg gctcagcctg ccccagccgg tgtatctccg ccgcttcagc tgcttcatca
      781 ctttgtcgtc gaacaggtgg gcataggttt tcagccgttc ctcgatcatc tctctgtcct
      841 caaacagtgt cagggtcagc acgatatctt ccagaatgtc ct
//
