LOCUS       sequence-82064-e        7316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of human LIN-41 / TRIM71.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Worringer KA, Rand TA, Hayashi Y, Sami S, Takahashi K, Tanabe K, 
            Narita M, Srivastava D, Yamanaka S
  TITLE     The let-7/LIN-41 pathway regulates reprogramming to human induced 
            pluripotent stem cells by controlling expression of 
            prodifferentiation genes.
  JOURNAL   Cell Stem Cell. 2014 Jan 2;14(1):40-52. doi: 
            10.1016/j.stem.2013.11.001. Epub 2013 Nov 14.
  PUBMED    24239284
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.11.001"; journalName: "Cell Stem Cell"; date: 
            "2014-01-2- 2"; volume: "14"; issue: "1"; pages: "40-52"
COMMENT     Sequence Label: pMXs-hs-LIN-41
FEATURES             Location/Qualifiers
     source          1..7316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     1979..1995
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1979..1995
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     LTR             4821..5290
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5420..5437)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5591..6179)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5671..5690)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6343..7134,7135..7203))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6966..6985
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7204..7308)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga attcctgcag gcctcgaggg ccggcgcgcc gcggccgctc
     1981 tagaactagt ggatcccccg ggctgcagga attcggcacg agctgggttg caaatggctt
     2041 cgttccccga gaccgatttc cagatctgct tgctgtgcaa ggagatgtgc ggctcgccgg
     2101 cgccgctctc ctccaactcg tccgcgtcgt cgtcctcctc gcagacgtcc acgtcgtcgg
     2161 ggggcggcgg cgggggccct ggggcggcgg cgcgccgcct acacgtcctg ccctgcctgc
     2221 acgccttctg ccgcccctgc ctcgaggcgc accggctgcc ggcggcgggc ggcggcgcgg
     2281 cgggagagcc gctcaagctg cgctgccccg tgtgcgacca gaaagtagtg ctagccgagg
     2341 cggcgggtat ggacgcgctg ccttcgtccg ccttcctgct tagcaacctg ctcgacgcgg
     2401 tggtggccac tgccgacgag ccgccgccca agaacgggcg cgccggcgct ccggcgggag
     2461 cgggcggcca cagcaaccac cggcaccacg ctcaccacgc gcacccgcgc gcgtccgcct
     2521 ccgcgccgcc actcccgcag gcgccgcagc cgcccgcgcc ttcccgctcg gcacccggcg
     2581 gccctgccgc ttccccgtcg gcgctgctgc tccgccgtcc tcacggctgc agctcgtgcg
     2641 atgagggcaa cgcagcttct tcgcgctgcc tcgactgcca ggagcacctg tgcgacaact
     2701 gcgtccgagc gcaccagcgc gtgcgcctca ccaaggacca ctacatcgag cgcggcccgc
     2761 cgggtcccgg tgccgcagca gcggcgcagc agctcgggct cgggccgccc tttcccggcc
     2821 cgcccttctc catcctctca gtgtttcccg agcgcctcgg cttctgccag caccacgacg
     2881 acgaggtgct gcacctgtac tgtgacactt gctctgtacc catctgtcgt gagtgcacaa
     2941 tgggccggca tgggggccac agcttcatct acctccagga ggcactgcag gactcacggg
     3001 cactcaccat ccagctgctg gcagatgccc agcagggacg acaggcaatc cagctgagca
     3061 tcgagcaggc ccagacggtg gcggaacagg tggagatgaa ggcgaaggtt gtgcagtcgg
     3121 aggtcaaagc cgtgacggcg aggcataaga aagccctgga ggaacgcgag tgtgagctgc
     3181 tgtggaaggt agaaaagatc cgccaggtga aagccaagtc tctgtacctg caggtggaga
     3241 agctgcggca aaacctcaac aagcttgaga gcaccatcag tgccgtgcag caggtcctgg
     3301 aggagggtag agcgctagac atcctactgg cccgagaccg gatgctggcc caggtgcagg
     3361 agctgaagac cgtgcggagc ctcctgcagc cccaggaaga cgaccgagtc atgttcacac
     3421 cccccgatca ggcactgtac cttgccatca agtcttttgg ctttgttagc agcggggcct
     3481 ttgccccact caccaaggcc acaggcgatg gcctcaagcg tgccctccag ggtaaggtgg
     3541 cctccttcac agtcattggt tatgaccacg atggtgagcc ccgcctctca ggaggcgacc
     3601 tgatgtcggc tgtggtcctg ggccctgatg gcaacctgtt tggtgcagag gtgagtgatc
     3661 agcagaatgg gacatacgtg gtgagttacc gaccccagct ggagggtgag cacctggtat
     3721 ctgtgacact gtgcaaccag cacattgaga acagcccttt caaggtggtg gtcaagtcag
     3781 gccgcagcta cgtgggcatt gggctcccgg gcctgagctt cggcagtgag ggtgacagcg
     3841 atggcaagct ctgccgccct tggggtgtga gtgtagacaa ggagggctac atcattgtcg
     3901 ccgaccgcag caacaaccgc atccaggtgt tcaagccctg cggcgccttc caccacaaat
     3961 tcggcaccct gggctcccgg cctgggcagt tcgaccgacc agccggcgtg gcctgtgacg
     4021 cctcacgcag gatcgtggtg gctgacaagg acaatcatcg catccagatc ttcacgttcg
     4081 agggccagtt cctcctcaag tttggtgaga aaggaaccaa gaatgggcag ttcaactacc
     4141 cttgggatgt ggcggtgaat tctgagggca agatcctggt ctcagacacg aggaaccacc
     4201 ggatccagct gtttgggcct gatggtgtct tcctaaacaa gtatggcttc gagggggctc
     4261 tctggaagca ctttgactcc ccacggggtg tggccttcaa ccatgagggc cacttggtgg
     4321 tcactgactt caacaaccac cggctcctgg ttattcaccc cgactgccag tcggcacgct
     4381 ttctgggctc ggagggcaca ggcaatgggc agttcctgcg cccacaaggg gtagctgtgg
     4441 accaggaagg gcgcatcatt gtggcggatt ccaggaacca tcgggtacag atgtttgaat
     4501 ccaacggcag cttcctgtgc aagtttggtg ctcaaggcag cggctttggg cagatggacc
     4561 gcccttccgg catcgccatc acccccgacg gaatgatcgt tgtggtggac tttggcaaca
     4621 atcgaatcct cgtcttctaa ttgcatttcc taggacgata aaataaaaga ttttatttag
     4681 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     4741 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag
     4801 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     4861 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     4921 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     4981 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     5041 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     5101 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
     5161 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     5221 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     5281 gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca
     5341 ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5401 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5461 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5521 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5581 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5641 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5701 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5761 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     5821 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5881 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     5941 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6001 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     6061 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6121 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6181 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6241 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg cggccggccg
     6301 caaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6361 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6421 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6481 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6541 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6601 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6661 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6721 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6781 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6841 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6901 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6961 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7021 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7081 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7141 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7201 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7261 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttc
//
