LOCUS       sequence-82068-e        6954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of human LIN-41 / TRIM71 containing deletion 
            of the BBox domain and 3 N-terminal HA tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Worringer KA, Rand TA, Hayashi Y, Sami S, Takahashi K, Tanabe K, 
            Narita M, Srivastava D, Yamanaka S
  TITLE     The let-7/LIN-41 pathway regulates reprogramming to human induced 
            pluripotent stem cells by controlling expression of 
            prodifferentiation genes.
  JOURNAL   Cell Stem Cell. 2014 Jan 2;14(1):40-52. doi: 
            10.1016/j.stem.2013.11.001. Epub 2013 Nov 14.
  PUBMED    24239284
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.11.001"; journalName: "Cell Stem Cell"; date: 
            "2014-01-2- 2"; volume: "14"; issue: "1"; pages: "40-52"
COMMENT     Sequence Label: pMXs-hs-3xHA-deltaBBox-LIN-41
FEATURES             Location/Qualifiers
     source          1..6954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1951..1960
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1963..2052
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     LTR             4459..4928
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5058..5075)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5229..5817)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5309..5328)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5981..6772,6773..6841))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6604..6623
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6842..6946)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga attcctgcag gccaccatgg catacccata cgatgttcct
     1981 gactatgcgg gctatcccta tgacgtcccg gactatgcag gatcctatcc atatgacgtt
     2041 ccagattacg ctatggcttc gttccccgag accgatttcc agatctgctt gctgtgcaag
     2101 gagatgtgcg gctcgccggc gccgctctcc tccaactcgt ccgcgtcgtc gtcctcctcg
     2161 cagacgtcca cgtcgtcggg gggcggcggc gggggccctg gggcggcggc gcgccgccta
     2221 cacgtcctgc cctgcctgca cgccttctgc cgcccctgcc tcgaggcgca ccggctgccg
     2281 gcggcgggcg gcggcgcggc gggagagccg ctcaagctgc gctgccccgt gtgcgaccag
     2341 aaagtagtgc tagccgaggc ggcgggtatg gacgcgctgc cttcgtccgc cttcctgctt
     2401 agcaacctgc tcgacgcggt ggtggccact gccgacgagc cgccgcccaa gaacgggcgc
     2461 gccggcgctc cggcgggagc gggcggccac agcaaccacc ggcaccacgc tcaccacgcg
     2521 cacccgcgcg cgtccgcctc cgcgccgcca ctcccgcagg cgccgcagcc gcccgcgcct
     2581 tcccgctcgg cacccggcgg ccctgccgct tccccgtcgg cgctgctgct ctcacgggca
     2641 ctcaccatcc agctgctggc agatgcccag cagggacgac aggcaatcca gctgagcatc
     2701 gagcaggccc agacggtggc ggaacaggtg gagatgaagg cgaaggttgt gcagtcggag
     2761 gtcaaagccg tgacggcgag gcataagaaa gccctggagg aacgcgagtg tgagctgctg
     2821 tggaaggtag aaaagatccg ccaggtgaaa gccaagtctc tgtacctgca ggtggagaag
     2881 ctgcggcaaa acctcaacaa gcttgagagc accatcagtg ccgtgcagca ggtcctggag
     2941 gagggtagag cgctagacat cctactggcc cgagaccgga tgctggccca ggtgcaggag
     3001 ctgaagaccg tgcggagcct cctgcagccc caggaagacg accgagtcat gttcacaccc
     3061 cccgatcagg cactgtacct tgccatcaag tcttttggct ttgttagcag cggggccttt
     3121 gccccactca ccaaggccac aggcgatggc ctcaagcgtg ccctccaggg taaggtggcc
     3181 tccttcacag tcattggtta tgaccacgat ggtgagcccc gcctctcagg aggcgacctg
     3241 atgtcggctg tggtcctggg ccctgatggc aacctgtttg gtgcagaggt gagtgatcag
     3301 cagaatggga catacgtggt gagttaccga ccccagctgg agggtgagca cctggtatct
     3361 gtgacactgt gcaaccagca cattgagaac agccctttca aggtggtggt caagtcaggc
     3421 cgcagctacg tgggcattgg gctcccgggc ctgagcttcg gcagtgaggg tgacagcgat
     3481 ggcaagctct gccgcccttg gggtgtgagt gtagacaagg agggctacat cattgtcgcc
     3541 gaccgcagca acaaccgcat ccaggtgttc aagccctgcg gcgccttcca ccacaaattc
     3601 ggcaccctgg gctcccggcc tgggcagttc gaccgaccag ccggcgtggc ctgtgacgcc
     3661 tcacgcagga tcgtggtggc tgacaaggac aatcatcgca tccagatctt cacgttcgag
     3721 ggccagttcc tcctcaagtt tggtgagaaa ggaaccaaga atgggcagtt caactaccct
     3781 tgggatgtgg cggtgaattc tgagggcaag atcctggtct cagacacgag gaaccaccgg
     3841 atccagctgt ttgggcctga tggtgtcttc ctaaacaagt atggcttcga gggggctctc
     3901 tggaagcact ttgactcccc acggggtgtg gccttcaacc atgagggcca cttggtggtc
     3961 actgacttca acaaccaccg gctcctggtt attcaccccg actgccagtc ggcacgcttt
     4021 ctgggctcgg agggcacagg caatgggcag ttcctgcgcc cacaaggggt agctgtggac
     4081 caggaagggc gcatcattgt ggcggattcc aggaaccatc gggtacagat gtttgaatcc
     4141 aacggcagct tcctgtgcaa gtttggtgct caaggcagcg gctttgggca gatggaccgc
     4201 ccttccggca tcgccatcac ccccgacgga atgatcgttg tggtggactt tggcaacaat
     4261 cgaatcctcg tcttctaatt gcatttccta ggacgataaa ataaaagatt ttatttagtc
     4321 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa
     4381 cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt
     4441 caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
     4501 gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg
     4561 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca
     4621 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     4681 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     4741 ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga
     4801 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
     4861 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
     4921 gtctttcaca tgcagcatgt atcaaaatta atttggtttt ttttcttaag tatttacatt
     4981 aaatggccat agttgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5041 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5101 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5161 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5221 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5281 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5341 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5401 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5461 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5521 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5581 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5641 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5701 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5761 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5821 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5881 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttgcg gccggccgca
     5941 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6001 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6061 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6121 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6181 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6241 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6301 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6361 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6421 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6481 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6541 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6601 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6661 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6721 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6781 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6841 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6901 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttc
//
