LOCUS       sequence-82076-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The expression vector for humanized nickase Cas9 (Cas9D10A) under 
            CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     pCAG-hCas9D10A
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCAG-hCas9D10A
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgcagtcgac ggtaccgcca ccatggacaa gaagtactcc attgggctcg ctatcggcac
       61 aaacagcgtc ggctgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa
      121 agttctgggc aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt
      181 cgactccggg gagacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac
      241 ccgcagaaag aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt
      301 ggatgactct ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca
      361 cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc
      421 aaccatatat catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt
      481 gatctatctc gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga
      541 cctgaaccca gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa
      601 tcagcttttc gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag
      661 cgctaggctg tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa
      721 gaagaacggc ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa
      781 atctaacttc gacctggccg aagatgccaa gcttcaactg agcaaagaca cctacgatga
      841 tgatctcga
//
