LOCUS       sequence-82077-e        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  P2A peptide from porcine teschovirus-1 flanked by restriction sites 
            in shuttle vector for easy cloning of multiple separate proteins to 
            be expressed from single message.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Daniels RW, Rossano AJ, Macleod GT, Ganetzky B
  TITLE     Expression of multiple transgenes from a single construct using 
            viral 2A peptides in Drosophila.
  JOURNAL   PLoS One. 2014 Jun 19;9(6):e100637. doi: 
            10.1371/journal.pone.0100637. eCollection 2014.
  PUBMED    24945148
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-06-19"; volume: "9(6):e100637. doi"; pages: " 
            10.1371/journal.pone.0100637. eCollection 2014"
COMMENT     Sequence Label: pC5Kan-P2A
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             222..278
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(613..632)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 cctttgatct tttctactga acggtgatcc ccaccggaat tgcggcctca gcagattaat
       61 ctggccggcc tacttaatta agtggaattc aaaggtaccc gcccgggatc agatccgcgg
      121 ccgcataggc cactagtgga tctggatcct tcttaagaag agctctaaag ctttatctag
      181 agtcgacctc gaacgttaac tcgaggccta taagatccgg cgccaccaac ttctccctgc
      241 tgaagcaggc cggcgacgtg gaggagaacc ccggccccgc ccccagatct cttgcatact
      301 acctgcagga taccggtata acgcgtatag ctagctttcc taggtctgcg atcgcaaagg
      361 cgcgccatag tttaaacaag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
      421 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
      481 tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
      541 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
      601 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
      661 cagacggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
      721 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatacgcg ccttgagcct
      781 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
      841 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
      901 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
      961 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcc
//
