LOCUS       sequence-82083-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of a Nano-lantern-based luminescent Ca2+ 
            indicator with Kd=600nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(Ca2+)_600/pcDNA3
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(314..391)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 gccctctaga tgcatgctcg agcggccgcc agtgtgatgg atatctgcag aattcttact
       61 gctcgttctt cagcactctc tccacgaagc tcttgatgta cttgcccatc tcgtcggggg
      121 cgtcctcctg gaggaagtgc aggcccttca ccttcacgaa ctcggtgttg gggaacttct
      181 tggcgccctc cacgatggcg ttgctgaaga agccggggtc accctcgatg aacagcttgg
      241 gcaggtcgtc gctggctctc aggtaggcgt tgtagtttct cacgatctgc accacgtcgg
      301 gcttgccgag ctccagtgcc ccggagctgg agatcttctt gaaccggttg gcagcgctga
      361 cggcaatgaa gtttttcttc cagcgcctct tccccccctt tgctgtcatc atttgtacaa
      421 actcttcata gtttacttgg ccatcaccat cgatatctgc ttcccttatc atttcatcaa
      481 cttcttcatc tgttaacttc tccccgaggt ttgtcatgac gtgacgtaac tgagcagcgc
      541 tgatgtagcc gttcccatcc ttgtcaaaaa cacggaatgc ttctcggatt tcctcttcgc
      601 tgtctgtgtc cttcattttt ctagccatca tagtaagaaa ttcaggaaag taaatcgttc
      661 cattgccatc agcatcgact tcattgatca tatcctgcaa ttctgcttcc gttgggtttt
      721 gtccaagcga cctcataacg gtgccaagtt cctttgtggt gatggtgccg tccccatcct
      781 tgtcgaataa tgagaaggct tctttgaact ctgcaatctg ctcttctgtc agttggtcat
      841 gcatccatgg gcccttcacc aggg
//
