LOCUS       sequence-82086-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of a Nano-lantern-based luminescent Ca2+ 
            indicator with Kd=300nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(Ca2+)_300/pcDNA3
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(318..395)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 tagggccctc tagatgcatg ctcgagcggc cgccagtgtg atggatatct gcagaattct
       61 tactgctcgt tcttcagcac tctctccacg aagctcttga tgtacttgcc catctcgtcg
      121 ggggcgtcct cctggaggaa gtgcaggccc ttcaccttca cgaactcggt gttggggaac
      181 ttcttggcgc cctccacgat ggcgttgctg aagaagccgg ggtcaccctc gatgaacagc
      241 ttgggcaggt cgtcgctggc tctcaggtag gcgttgtagt ttctcacgat ctgcaccacg
      301 tcgggcttgc cgagctccag tgccccggag ctggagatct tcttgaaccg gttggcagcg
      361 ctgacggcaa tgaagttttt cttccagcgc ctcttggacc cgcccccctt tgctgtcatc
      421 atttgtacaa actcttcata gtttacttgg ccatcaccat cgatatctgc ttcccttatc
      481 atttcatcaa cttcttcatc tgttaacttc tccccgaggt ttgtcatgac gtgacgtaat
      541 tgagcagcgc tgatgtagcc gttcccatcc ttgtcaaaaa cacggaatgc ttctcggatt
      601 tcctcttcgc tgtctgtgtc cttcattttt ctagccatca tagtaagaaa ttcaggaaag
      661 taaatcgttc cattgccatc agcatcgact tcattgatca tatcctgcaa ttctgcttcc
      721 gttgggtttt gtccaagcga cctcataacg gtgccaagtt cctttgtggt gatggtgccg
      781 tccccatcct tgtcgaataa tgagaaggct tctt
//
