LOCUS       sequence-82088-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of a Nano-lantern-based luminescent Ca2+ 
            indicator with Kd=150nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern(Ca2+)_150/pcDNA3
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(319..396)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 tagggccctc tagatgcatg gctcgagcgg ccgccagtgt gatggatatc tgcagaattc
       61 ttactgctcg ttcttcagca ctctctccac gaagctcttg atgtacttgc ccatctcgtc
      121 gggggcgtcc tcctggagga agtgcaggcc cttcaccttc acgaactcgg tgttggggaa
      181 cttcttggcg ccctccacga tggcgttgct gaagaagccg gggtcaccct cgatgaacag
      241 cttgggcagg tcgtcgctgg ctctcaggta ggcgttgtag tttctcacga tctgcaccac
      301 gtcgggcttg ccgagctcca gtgccccgga gctggagatc ttcttgaacc ggttggcagc
      361 gctgacggca atgaagtttt tcttccagcg cctcttcccc ccctttgctg tcatcatttg
      421 tacaaactct tcatagttta cttggccatc accatcgata tctgcttccc ttatcatttc
      481 atcaacttct tcatctgtta acttctcccc gaggtttgtc atgacgtgac gtaattcagc
      541 agcgctgatg tagccgttcc tatccttgtc aaaaacacgg aatgcttctc ggatttcctc
      601 ttcgctgtct gtgtccttca tttttctagc catcatagta agaaattcag gaaagtaaat
      661 cgttccattg ccatcagcat cgacttcatt gatcatatcc tgcaattctg cttccgttgg
      721 gttttgtcca agcgacctca taacggtgcc aagttccttt gtggtgatgg tgccgtcccc
      781 atcct
//
