LOCUS       sequence-82095-e        7335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of human LIN-41 / TRIM71 containing cysteine 
            to alanine point mutations of all 7 cysteines in the RING domain and
            3 N-terminal HA tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Worringer KA, Rand TA, Hayashi Y, Sami S, Takahashi K, Tanabe K, 
            Narita M, Srivastava D, Yamanaka S
  TITLE     The let-7/LIN-41 pathway regulates reprogramming to human induced 
            pluripotent stem cells by controlling expression of 
            prodifferentiation genes.
  JOURNAL   Cell Stem Cell. 2014 Jan 2;14(1):40-52. doi: 
            10.1016/j.stem.2013.11.001. Epub 2013 Nov 14.
  PUBMED    24239284
REFERENCE   2  (bases 1 to 7335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.11.001"; journalName: "Cell Stem Cell"; date: 
            "2014-01-2- 2"; volume: "14"; issue: "1"; pages: "40-52"
COMMENT     Sequence Label: pMXs-hs-3xHA-7CtoA-LIN-41
FEATURES             Location/Qualifiers
     source          1..7335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1951..1960
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1963..2052
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     LTR             4840..5309
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5439..5456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5610..6198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5690..5709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6362..7153,7154..7222))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6985..7004
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7223..7327)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga attcctgcag gccaccatgg catacccata cgatgttcct
     1981 gactatgcgg gctatcccta tgacgtcccg gactatgcag gatcctatcc atatgacgtt
     2041 ccagattacg ctatggcttc gttccccgag accgatttcc agatcgcctt gctggccaag
     2101 gagatgtgcg gctcgccggc gccgctctcc tccaactcgt ccgcgtcgtc gtcctcctcg
     2161 cagacgtcca cgtcgtcggg gggcggcggc gggggccctg gggcggcggc gcgccgccta
     2221 cacgtcctgc ccgccctgca cgccttcgcc cgccccgccc tcgaggcgca ccggctgccg
     2281 gcggcgggcg gcggcgcggc gggagagccg ctcaagctgc gcgcccccgt ggccgaccag
     2341 aaagtagtgc tagccgaggc ggcgggtatg gacgcgctgc cttcgtccgc cttcctgctt
     2401 agcaacctgc tcgacgcggt ggtggccact gccgacgagc cgccgcccaa gaacgggcgc
     2461 gccggcgctc cggcgggagc gggcggccac agcaaccacc ggcaccacgc tcaccacgcg
     2521 cacccgcgcg cgtccgcctc cgcgccgcca ctcccgcagg cgccgcagcc gcccgcgcct
     2581 tcccgctcgg cacccggcgg ccctgccgct tccccgtcgg cgctgctgct ccgccgtcct
     2641 cacggctgca gctcgtgcga tgagggcaac gcagcttctt cgcgctgcct cgactgccag
     2701 gagcacctgt gcgacaactg cgtccgagcg caccagcgcg tgcgcctcac caaggaccac
     2761 tacatcgagc gcggcccgcc gggtcccggt gccgcagcag cggcgcagca gctcgggctc
     2821 gggccgccct ttcccggccc gcccttctcc atcctctcag tgtttcccga gcgcctcggc
     2881 ttctgccagc accacgacga cgaggtgctg cacctgtact gtgacacttg ctctgtaccc
     2941 atctgtcgtg agtgcacaat gggccggcat gggggccaca gcttcatcta cctccaggag
     3001 gcactgcagg actcacgggc actcaccatc cagctgctgg cagatgccca gcagggacga
     3061 caggcaatcc agctgagcat cgagcaggcc cagacggtgg cggaacaggt ggagatgaag
     3121 gcgaaggttg tgcagtcgga ggtcaaagcc gtgactgcga ggcataagaa agccctggag
     3181 gaacgcgagt gtgagctgct gtggaaggta gaaaagatcc gccaggtgaa agccaagtct
     3241 ctgtacctgc aggtggagaa gctgcggcaa aacctcaaca agcttgagag caccatcagt
     3301 gccgtgcagc aggtcctgga ggagggtaga gcgctagaca tcctactggc ccgagaccgg
     3361 atgctggccc aggtgcagga gctgaagacc gtgcggagcc tcctgcagcc ccaggaagac
     3421 gaccgagtca tgttcacacc ccccgatcag gcactgtacc ttgccatcaa gtcttttggc
     3481 tttgttagca gcggggcctt tgccccactc accaaggcca caggcgatgg cctcaagcgt
     3541 gccctccagg gtaaggtggc ctccttcaca gtcattggtt atgaccacga tggtgagccc
     3601 cgcctctcag gaggcgacct gatgtcggct gtggtcctgg gccctgatgg caacctgttt
     3661 ggtgcagagg tgagtgatca gcagaatggg acatacgtgg tgagttaccg accccagctg
     3721 gagggtgagc acctggtatc tgtgacactg tgcaaccagc acattgagaa cagccctttc
     3781 aaggtggtgg tcaagtcagg ccgcagctac gtgggcattg ggctcccggg cctgagcttc
     3841 ggcagtgagg gtgacagcga tggcaagctc tgccgccctt ggggtgtgag tgtagacaag
     3901 gagggctaca tcattgtcgc cgaccgcagc aacaaccgca tccaggtgtt caagccctgc
     3961 ggcgccttcc accacaaatt cggcaccctg ggctcccggc ctgggcagtt cgaccgacca
     4021 gccggcgtgg cctgtgacgc ctcacgcagg atcgtggtgg ctgacaagga caatcatcgc
     4081 atccagatct tcacgttcga gggccagttc ctcctcaagt ttggtgagaa aggaaccaag
     4141 aatgggcagt tcaactaccc ttgggatgtg gcggtgaatt ctgagggcaa gatcctggtc
     4201 tcagacacga ggaaccaccg gatccagctg tttgggcctg atggtgtctt cctaaacaag
     4261 tatggcttcg agggggctct ctggaagcac tttgactccc cacggggtgt ggccttcaac
     4321 catgagggcc acttggtggt cactgacttc aacaaccacc ggctcctggt tattcacccc
     4381 gactgccagt cggcacgctt tctgggctcg gagggcacag gcaatgggca gttcctgcgc
     4441 ccacaagggg tagctgtgga ccaggaaggg cgcatcattg tggcggattc caggaaccat
     4501 cgggtacaga tgtttgaatc caacggcagc ttcctgtgca agtttggtgc tcaaggcagc
     4561 ggctttgggc agatggaccg cccttccggc atcgccatca cccccgacgg aatgatcgtt
     4621 gtggtggact ttggcaacaa tcgaatcctc gtcttctaat tgcatttcct aggacgataa
     4681 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     4741 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa
     4801 tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga
     4861 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat
     4921 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     4981 atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag
     5041 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     5101 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     5161 ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
     5221 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     5281 attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt aatttggttt
     5341 tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg ccaacgcgcg
     5401 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5461 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5521 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5581 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5641 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5701 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5761 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5821 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5881 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5941 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6001 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6061 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6121 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6181 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6241 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6301 atccttttgc ggccggccgc aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6361 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6421 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6481 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6541 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6601 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6661 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6721 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6781 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6841 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6901 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6961 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7021 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7081 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7141 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7201 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7261 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7321 ttccgcgcac atttc
//
