LOCUS       sequence-8212-ex        2167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Kaykas A, Moon RT
  TITLE     A plasmid-based system for expressing small interfering RNA 
            libraries in mammalian cells.
  JOURNAL   BMC Cell Biol. 2004 Apr 30;5:16. doi: 10.1186/1471-2121-5-16.
  PUBMED    15119963
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Cell 
            Biol."; date: "2004-04-30"; volume: "5:16. doi"; pages: " 
            10.1186/1471-2121-5-16"
COMMENT     Sequence Label: V43 pHIPPY GFP22
FEATURES             Location/Qualifiers
     source          1..2167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(42..630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(122..141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     690..709
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(858..877)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(1028..1048)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        1139..1436
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        1237..1447
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1306..1441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        1452..1499
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1518..1892
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(1922..1941)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1966..2013
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 caattcgcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
       61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      481 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      541 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      601 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      661 gacgctcagt gggcggccgc atttgcatgt cgctatgtgt tctgggaaat caccataaac
      721 gtgaaatgtc tttggatttg ggaatcttat aagtggatcc tgagaccgtc tcaaaaagca
      781 agctgaccct gaagttcatt ttttgagacg ctagccacaa gatatataaa gccaagaaat
      841 cgaaatactt tcaagttacg gtaagcatat gatagtccat tttaaaacat aattttaaaa
      901 ctgcaaacta cccaagaaat tattactttc tacgtcacgt attttgtact aatatctttg
      961 tgtttacagt caaattaatt ctaattatct ctctaacagc cttgtatcgt atatgcaaat
     1021 atgaaggaat catgggaaat aggccctctt cctgcccgac cttggcgcgc gctcggcgcg
     1081 cggtcacgct ccgtcacgtg gtgcgttttg cctgcgcgtc tttccactgg ggacgcgtgg
     1141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     1201 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     1261 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     1321 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     1381 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     1441 ctagtctcga tgttgacaat taatcatcgg catagtatat cggcatagta taatacgacg
     1501 gatcaaggag gtcgaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     1561 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     1621 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     1681 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     1741 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     1801 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     1861 gcgtgcactt cgtggccgag gagcaggact gaactagcga tatcactagg gggaggctaa
     1921 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     1981 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     2041 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     2101 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     2161 cggcagg
//
