LOCUS       sequence-8213-ex        2145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Kaykas A, Moon RT
  TITLE     A plasmid-based system for expressing small interfering RNA 
            libraries in mammalian cells.
  JOURNAL   BMC Cell Biol. 2004 Apr 30;5:16. doi: 10.1186/1471-2121-5-16.
  PUBMED    15119963
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Cell 
            Biol."; date: "2004-04-30"; volume: "5:16. doi"; pages: " 
            10.1186/1471-2121-5-16"
COMMENT     Sequence Label: V44 pHIPPY blunt
FEATURES             Location/Qualifiers
     source          1..2145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(42..630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(122..141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     690..709
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(836..855)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(1006..1026)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        1117..1414
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        1215..1425
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1284..1419
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        1430..1477
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1496..1870
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(1900..1919)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1944..1991
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 caattcgcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
       61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      481 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      541 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      601 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      661 gacgctcagt gggcggccgc atttgcatgt cgctatgtgt tctgggaaat caccataaac
      721 gtgaaatgtc tttggatttg ggaatcttat aagtggatcc tgagaccgtc tcaaaaattt
      781 ttgagacgct agccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt
      841 aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta
      901 ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattct
      961 aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag
     1021 gccctcttcc tgcccgacct tggcgcgcgc tcggcgcgcg gtcacgctcc gtcacgtggt
     1081 gcgttttgcc tgcgcgtctt tccactgggg acgcgtggtg tggaaagtcc ccaggctccc
     1141 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     1201 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     1261 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     1321 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     1381 agctattcca gaagtagtga ggaggctttt ttggaggcct agtctcgatg ttgacaatta
     1441 atcatcggca tagtatatcg gcatagtata atacgacgga tcaaggaggt cgaccatggc
     1501 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     1561 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     1621 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     1681 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     1741 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     1801 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     1861 gcaggactga actagcgata tcactagggg gaggctaact gaaacacgga aggagacaat
     1921 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     1981 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     2041 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     2101 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcagg
//
