LOCUS       sequence-8214-ex        2164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Kaykas A, Moon RT
  TITLE     A plasmid-based system for expressing small interfering RNA 
            libraries in mammalian cells.
  JOURNAL   BMC Cell Biol. 2004 Apr 30;5:16. doi: 10.1186/1471-2121-5-16.
  PUBMED    15119963
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Cell 
            Biol."; date: "2004-04-30"; volume: "5:16. doi"; pages: " 
            10.1186/1471-2121-5-16"
COMMENT     Sequence Label: V45 pHIPPY PGL3 Luciferase
FEATURES             Location/Qualifiers
     source          1..2164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(42..630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(122..141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     690..709
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(855..874)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(1025..1045)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        1136..1433
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        1234..1444
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1303..1438
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        1449..1496
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1515..1889
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(1919..1938)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1963..2010
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 caattcgcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
       61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      481 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      541 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      601 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      661 gacgctcagt gggcggccgc atttgcatgt cgctatgtgt tctgggaaat caccataaac
      721 gtgaaatgtc tttggatttg ggaatcttat aagtggatcc tgagaccgtc tcaaaaaggc
      781 tcctcagaaa cagctctttt tgagacgcta gccacaagat atataaagcc aagaaatcga
      841 aatactttca agttacggta agcatatgat agtccatttt aaaacataat tttaaaactg
      901 caaactaccc aagaaattat tactttctac gtcacgtatt ttgtactaat atctttgtgt
      961 ttacagtcaa attaattcta attatctctc taacagcctt gtatcgtata tgcaaatatg
     1021 aaggaatcat gggaaatagg ccctcttcct gcccgacctt ggcgcgcgct cggcgcgcgg
     1081 tcacgctccg tcacgtggtg cgttttgcct gcgcgtcttt ccactgggga cgcgtggtgt
     1141 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     1201 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     1261 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     1321 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     1381 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     1441 gtctcgatgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacggat
     1501 caaggaggtc gaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     1561 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     1621 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     1681 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     1741 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     1801 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     1861 tgcacttcgt ggccgaggag caggactgaa ctagcgatat cactaggggg aggctaactg
     1921 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     1981 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     2041 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     2101 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     2161 cagg
//
