LOCUS       sequence-82183-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral bicistronic expression of p18Ink4c and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Forget A, Ayrault O, den Besten W, Kuo ML, Sherr CJ, Roussel MF
  TITLE     Differential post-transcriptional regulation of two Ink4 proteins, 
            p18 Ink4c and p19 Ink4d.
  JOURNAL   Cell Cycle. 2008 Dec;7(23):3737-46. Epub 2008 Dec 13.
  PUBMED    19029828
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2008-12"; volume: "7(23)"; pages: "3737-46. Epub 
            2008 Dec 13"
COMMENT     Sequence Label: pMSCV-p18Ink4c-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(128..130,131..133,134..646)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(173..194)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(434..453)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgctgt ttagtcgagg
       61 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
      121 taataccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      181 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      241 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      301 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
      361 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      421 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      481 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      541 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      601 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      661 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      721 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      781 catggtcctg ctggagttc
//
