LOCUS       sequence-82186-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human immunoglobulin gamma 1 antibody expression vector (lambda 
            light chain) without variable regions. Enables Colony-PCR screening 
            of false-positive (PCR vector template) colonies .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, 
            Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
  TITLE     A tool kit for rapid cloning and expression of recombinant 
            antibodies.
  JOURNAL   Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
  PUBMED    25073855
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-07-30"; volume: "4:5885. doi"; pages: " 
            10.1038/srep05885"
COMMENT     Sequence Label: pVITRO1-dV-IgG1/lambda
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgcagtacaa agtgttaccc ctctagacct ggaaagacca ggcggagttt caagttgcac
       61 gttttgtgtc attggggaaa cctgctccta gctatgaaca ttctgtaggg gccactgtct
      121 tctccacggt gctcccttca tgcgtgacct ggcagctgta gcttctgtgg gacttccact
      181 gctcaggcgt caggctcaga tagctgctgg ccgcgtactt gttgttgctt tgtttggagg
      241 gtgtggtggt ctccactccc gccttgacgg ggctgctatc tgccttccag gccactgtca
      301 cggctcccgg gtagaagtca cttatgagac acaccagtgt ggccttgttg gcttgaagct
      361 cctcagagga gggcgggaac agagtgaccg agggcgccgc cttgggctga cctagctcga
      421 ggatatcgaa ttcagagtcg actccggatc cataagcaag gagtccgagg agaagcatca
      481 tccaggccat attgctttga attagcggtg gctttcacaa cacctggaaa cgggtttaga
      541 aaataccttt gaaccgctaa gaggcttgag aattagcctc gctccaaact caaaggggca
      601 aattccaaga atgacttcca gcgccaggct ggcctgacta gtctccaccc accaaatgtg
      661 aacaaactcc aacgccatta gatcccctcc ccccgccgcg acgagcggtg ctcaagagaa
      721 gcccgaggtg actatgcggc cgatagacca cggggtcaca ggaagcagcg gccggcgagg
      781 ggcaaggccc tcttcctttg tgtgggtgac tcacccgccc gctccaccgg gctgccgcgt
//
