LOCUS       sequence-82193-e        7233 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of TUG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Bogan JS, Hendon N, McKee AE, Tsao TS, Lodish HF
  TITLE     Functional cloning of TUG as a regulator of GLUT4 glucose 
            transporter trafficking.
  JOURNAL   Nature. 2003 Oct 16;425(6959):727-33.
  PUBMED    14562105
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2003-10-16"; volume: "425(6959)"; pages: "727-33"
COMMENT     Sequence Label: pBICD2-TUG
FEATURES             Location/Qualifiers
     source          1..7233
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             7..600
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    663..1016
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1081..1497
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1427..1449
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1465..1481
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1507..1881
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1819..1835
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(2209..2231)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    join(3207..3781,3782..3784,3785..3793)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3374..3391)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     LTR             4678..5271
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5337..5354)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5508..6096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5588..5607)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6260..7051,7052..7120))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6883..6902
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7121..7225)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcataatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
       61 caaggcatgg aaaaatacat aactgagaat agaaaagttc agatcaaggt caggaacaga
      121 tggaacagct gaatatgggc caaagcggat atctgtggta agcagttcct gccccggctc
      181 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      241 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
      301 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
      361 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
      421 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
      481 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
      541 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt
      601 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
      661 gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt
      721 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga
      781 actgacgagt tcggaacacc cggccgcaac cctgggagac gtcccaggtc gggggccgtt
      841 tttgtggccc gacctgagtc caaaaatccc gatcgttttg gactctttgg tgcacccccc
      901 ttagaggagg gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg
      961 tctgaatttt tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc
     1021 atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa tgtgggcccg
     1081 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
     1141 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
     1201 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
     1261 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
     1321 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
     1381 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
     1441 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc
     1501 atatggccat atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct
     1561 gacgtgacaa gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta
     1621 gtccagcacg aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg
     1681 gtggtacctc acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag
     1741 aacctagaac ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc
     1801 aaagtagacg gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg
     1861 ggtggaccat cctctagact gccggatccc agtgtggtgg tacgggaatt caagcttgat
     1921 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
     1981 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gacagatcca
     2041 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
     2101 aagccgtggc ggtctggtac cggtgggtga agaccagaaa cagcacctcg atctgagccg
     2161 cgatattgcc cagcgtttca acgcgctgta tggcgagatc gatcccgtcg ttttacaacg
     2221 tcgtgactgg gaaaaccctg gcgttaccca acttaatggc cttggaggac atcccccttt
     2281 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     2341 cctgaattgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct
     2401 ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc
     2461 acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgccgt
     2521 ttgttcccac ggagaatccg acgggttgtt actcgctcac attttaatgt tgatgaaagc
     2581 tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg
     2641 tggtgcaacg ggcgctgggt cggttacggg caagacagtc gtttggcgtc ttaatttgag
     2701 ctcgagcgca tatctacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgctggag
     2761 tgacgggagt tatcttgaag atcaagatat gtggcggatg agcgggattc cgagcgaaaa
     2821 cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagagtg ggcgcggcga
     2881 cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca gccatcgcca
     2941 tctgctgcac gcggaagaac cgacatggct gttatacgac ggtttccata tggggattgg
     3001 tggcgacgac tcctggagcc cgtcagtatc ggcggaattc cagctgagcg ccggtcgcta
     3061 ccattaccag ttggtctggt gtcaaaaata ataataaccg ggcaggccat gtctgcccgt
     3121 atttcgcgta aggaaatcca ttatgtacta tttaaactcg agcggccgcc agcacagtgg
     3181 ccatggcatc tagggcggcc aattcgcccc tctccctccc ccccccctaa cgttactggc
     3241 cgaagccgct tggaataagg ccggtgtgtg tttgtctata tgtgattttc caccatattg
     3301 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     3361 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     3421 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     3481 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     3541 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     3601 tggctctcct caagcgtagt caacaagggg ctgaaggatg cccagaaggt accccattgt
     3661 atgggaatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     3721 aaagctctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     3781 gcttgccaca acccgcggta cgatcagctc atgaaatgta aattcctggg tagcttcttt
     3841 ctgctcttca gcctttccgg caaaggggcg gactgcagag acaatgagac catctggggt
     3901 gtcttgggtc atggcatcac cctgaacatc cccaactttc aaatgactga tgatattgat
     3961 gaggtgcgat gggtaaggag gggcaccctg gtcgcagagt ttaaaaggaa gaagccacct
     4021 tttttgatat cagaaacgta tgaggtctta gcaaacggat ccctgaagat aaagaagccg
     4081 atgatgagaa acgacagtgg cacctataat gtaatggtgt atggcacaaa tgggatgact
     4141 aggctggaga aggacctgga cgtgaggatt ctggagaggg tctcaaagcc catgatccac
     4201 tgggaatgcc ccaacacaac cctgacctgt gcggtcttgc aagggacaga ttttgaactg
     4261 aagctgtatc aaggggaaac actactcaat agtctccccc agaagaacat gagttaccag
     4321 tggaccaacc tgaacgcacc attcaagtgt gaggcgataa acccggtcag caaggagtct
     4381 aagatggaag ttgttaactg tccagagaaa ggtctgtcct tctatgtcac agtgggggtc
     4441 ggtgcaggag gactcctctt ggtgctcttg gtggcgcttt ttattttctg tatctgcaag
     4501 aggagaaaac ggaacaggag gagaaaagat gaagagctgg aaataaaagc ttccagaaca
     4561 agcactgtgg aaaggggccc caagccgcac tcaaccccag ccgcagcagc gcagaattaa
     4621 gcggccgtag tcgacgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     4681 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt ggaaggcatg
     4741 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc
     4801 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     4861 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     4921 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
     4981 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     5041 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     5101 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     5161 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     5221 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat tctgcattaa
     5281 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5341 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5401 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5461 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5521 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5581 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5641 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5701 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5761 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5821 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5881 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     5941 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6001 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6061 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6121 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6181 aaaaggatct tcacctagat ccttttgcgg ccggccgcaa atcaatctaa agtatatatg
     6241 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6301 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6361 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6421 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6481 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6541 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6601 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6661 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6721 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6781 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6841 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     6901 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6961 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7021 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7081 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7141 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7201 aaaataaaca aataggggtt ccgcgcacat ttc
//
