LOCUS       sequence-8220-ex        2304 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Nashmi R, Dickinson ME, McKinney S, Jareb M, Labarca C, Fraser SE, 
            Lester HA
  TITLE     Assembly of alpha4beta2 nicotinic acetylcholine receptors assessed 
            with functional fluorescently labeled subunits: effects of 
            localization, trafficking, and nicotine-induced upregulation in 
            clonal mammalian cells and in cultured midbrain neurons.
  JOURNAL   J Neurosci. 2003 Dec 17. 23(37):11554-67.
  PUBMED    14684858
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2003 Dec 17. 23(37):11554-67."
COMMENT     Sequence Label: nAChR beta2 CFP
FEATURES             Location/Qualifiers
     source          1..2304
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1167..1196
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1197..1199,1200..1202,1203..1913)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1242..1263)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1503..1522)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1850..1871
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctccgcgcta tgcaggcggc atggcccggt gctccaactc tatggcgctg ctgttcagct
       61 ttggcctcct ttggctgtgt tcaggggttt tgggtactga cacagaggag cggctggtgg
      121 agcatctctt ggatccttcc cgctataaca agctgatccg tccagctact aatggctctg
      181 agctggtgac tgtacagctc atggtatcat tggcacagct catcagtgtg cacgagcggg
      241 agcagatcat gaccaccaac gtctggctga cccaggagtg ggaagattat cgcctcacat
      301 ggaagcctga ggatttcgac aatatgaaga aagtccgact cccttctaag cacatctggc
      361 tcccagatgt ggttctatac aacaatgctg acggcatgta cgaagtctcc ttctattcca
      421 atgctgtggt ctcctatgat ggcagcatct tttggctacc gcctgccatc tacaagagcg
      481 catgcaagat tgaggtgaag cacttcccat ttgaccagca gaactgcacc atgaagttcc
      541 gctcctggac ctacgaccgc actgagattg acctggtgct caaaagcgat gtggccagcc
      601 tggacgactt cacacctagt ggggagtggg acatcatcgc actgccaggc cgacgcaacg
      661 agaacccaga cgactccacc tacgtggaca tcacctacga cttcatcatt cgtcgcaaac
      721 cgctcttcta caccatcaac ctcatcatcc cctgcgtact catcacctcg ctggccatcc
      781 tggtcttcta cctgccctca gactgtggtg aaaagatgac actttgtatt tctgtgctgc
      841 tggcgctcac ggtgttcctg ctgctcatct ccaagattgt gcctcccacc tccctcgacg
      901 taccgctggt gggaaagtac ctcatgttca ccatggtgct agtcaccttc tccatcgtca
      961 ctagcgtgtg tgtgctcaat gtgcaccacc gttcgcctac cacgcacacc atggcgccct
     1021 gggtcaaggt ggtcttcctg gagaagctgc ccaccctcct cttcctgcag cagccacgcc
     1081 accgctgtgc acgtcaacgc ctgcgcttgc gaaggcgcca gcgggaacgt gagggggcag
     1141 gcacactatt cttccgcaaa ggtcctgagc agaagctgat ctcagaggag gatctgatgg
     1201 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1261 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1321 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1381 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     1441 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1501 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1561 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1621 tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
     1681 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1741 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1801 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1861 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaaaggtc
     1921 ctgcagctga cccatgtacc tgctttgtca atcctgcatc aatgcagggc ttggctgggg
     1981 ctttccaggc tgagcccgct gcagccggtc tggggcgctc gatgggccca tgcagctgtg
     2041 gcctccggga agctgtggac ggtgtacgct tcattgcgga ccatatgcga agtgaagatg
     2101 atgaccagag tgtgagggag gattggaaat acgttgccat ggtgatcgac cgcctgttcc
     2161 tctggatctt tgtctttgtc tgtgtctttg ggaccattgg catgttcctg cagcctctct
     2221 tccagaacta cactgccacg accttccttc actctgacca ctcagctccc agctccaagt
     2281 gaggtctctc atttgcagct cctc
//
