LOCUS       sequence-82201-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter of membrane voltage with improved sensitivity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Hochbaum DR, Zhao Y, Farhi SL, Klapoetke N, Werley CA, Kapoor V, Zou
            P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, 
            Boulting GL, Straub C, Cho YK, Melkonian M, Wong GK, Harrison DJ, 
            Murthy VN, Sabatini BL, Boyden ES, Campbell RE, Cohen AE
  TITLE     All-optical electrophysiology in mammalian neurons using engineered 
            microbial rhodopsins.
  JOURNAL   Nat Methods. 2014 Aug;11(8):825-33. doi: 10.1038/nmeth.3000. Epub 
            2014 Jun 22.
  PUBMED    24952910
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3000"; journalName: "Nat Methods"; date: "2014-08"; 
            volume: "11"; issue: "8"; pages: "825-33"
COMMENT     Sequence Label: DRH334: FCK-QuasAr2-mO2
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(133..840)
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEYNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattctt attcattctc
       61 ataacaaaat ccacctacat ttatgtctat ttgatccagg gggatgtact cgccttcgct
      121 tgtgattcta ctcttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggcgcg
      181 ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca acttgatgtc
      241 gacgatgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc
      301 ggaggtgtag tggccgccgt ccttcagctt cagcctcatc ttgatcttgc ccttcagggc
      361 accgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat
      421 cacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc
      481 gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcgt agttcatcac
      541 gcgctcccac ttgaagccct cggggaagga cagcttgaag tagtcgggga tgtcggcggg
      601 gtgcttcacg taggccttgg agccgtaggt gaaatgaggg gacaggatgt cccaggcgaa
      661 gggcaggggg ccacccttgg tcaccttcag cttagcggtc tgaaagccct cgtaggggcg
      721 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgcgcac
      781 cttgaagcgc atgaactcct tgatgatggc catgttatac tcctcgccct tgctcaccat
      841 caacgtatc
//
