LOCUS       sequence-82207-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human Sumo1 with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Terui Y, Saad N, Jia S, McKeon F, Yuan J
  TITLE     Dual role of sumoylation in the nuclear localization and 
            transcriptional activation of NFAT1.
  JOURNAL   J Biol Chem. 2004 Jul 2. 279(27):28257-65.
  PUBMED    15117942
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2004 Jul 2. 279(27):28257-65."
COMMENT     Sequence Label: pcDNA3-HA-Sumo1
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             101..127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      585..965
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(672..691)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     883..904
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac catggcctcc tacccttatg atgtgccaga
      121 ttatgcctct cccgaattct gcagatatcc atcacactgg cggccgctcg agcggatgtc
      181 tgaccaggag gcaaaacctt caactgagga cttgggggat aagaaggaag gtgaatatat
      241 taaactcaaa gtcattggac aggatagcag tgagattcac ttcaaagtga aaatgacaac
      301 acatctcaag aaactcaaag aatcatactg tcaaagacag ggtgttccaa tgaattcact
      361 caggtttctc tttgagggtc agagaattgc tgataatcat actccaaaag aactgggaat
      421 ggaggaagaa gatgtgattg aagtttatca ggaacaaacg gggggtcatt caacagttta
      481 ggctctagag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
      541 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
      601 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      661 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      721 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
      781 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
      841 ggttttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
      901 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
      961 tttcg
//
