LOCUS       sequence-8221-ex        3151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Pagani F, Stuani C, Tzetis M, Kanavakis E, Efthymiadou A, 
            Doudounakis S, Casals T, Baralle FE
  TITLE     New type of disease causing mutations: the example of the composite 
            exonic regulatory elements of splicing in CFTR exon 12.
  JOURNAL   Hum Mol Genet. 2003 May 15. 12(10):1111-20.
  PUBMED    12719375
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2003 May 15. 12(10):1111-20."
COMMENT     Sequence Label: pTBNde(min)
FEATURES             Location/Qualifiers
     source          1..3151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(18..38)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        23..352
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      203..338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3118..3134)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3119..3135)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gagctcggta cccctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
       61 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      121 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      181 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      241 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
      301 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctcca
      361 agctctcgag gatcccccgg gctccgcgcc agccaatgag cgccgcccgg ccgggcgtgc
      421 ccccgcgccc caagcataaa ccctggcgcg ctcgcggccc ggcactcttc tggtccccac
      481 agactcagag agaacccacc atggtgctgt ctcctgccga caagaccaac gtcaaggccg
      541 cctggggtaa ggtcggcgcg cacgctggcg agtatggtgc ggaggccctg gagaggtgag
      601 gctccctccc ctgctccgac ccgggctcct cgcccgcccg gacccacagg ccaccctcaa
      661 ccgtcctggc cccggaccca aaccccaccc ctcactctgc ttctccccgc aggatgttcc
      721 tgtccttccc caccaccaag acctacttcc cgcacttcga cctgagccac ggctctgccc
      781 aggttaaggg ccacggcaag aaggtggccg acgccctgac caacgccgtg gcgcacgtgg
      841 acgacatgcc caacgcgctg tccgccctga gcgacctgca cgcgcacaag cttcgggtgg
      901 acccggtcaa cttcaaggtg agcggcgggc cgggagcgat ctgggtcgag gggcgagatg
      961 gcgccttcct cgcagggcag aggatcacgc gggttgcggg aggtgtagcg caggcggcgg
     1021 ctgcgggcct gggccctcgg ccccactgac cctcttctct gcacagctcc taagccactg
     1081 cctgctggtg acctgcactt ttgataacct gagtcccggc ctggagtaca atgtcagtgt
     1141 ttacactgtc aaggatgaca aggaaagtgt ccctatctct gataccatca tcccaggtaa
     1201 tagaaaaata agctgctatc ctgagagtga cacttccaat aagagtgggg attagcatct
     1261 taatccccag atgcttaagg gtgtcaacta tatttgggat ttaattccga tctcccagct
     1321 gcactttcca aaaccaagaa gtcaaagcag cgatttggac aaatgcttgc tgttaacact
     1381 gctttactgt ctgtgcttca ctgggatgct gtgtgttgca gcgagtatgt aatggagtgg
     1441 cagccatggc tttaactctg tattgtctgc tcacatggaa gtatgactaa aacactgtca
     1501 cgtgtctgta ctcagtactg ataggctcaa agtaatatgg taaatgcatc ccatcagtac
     1561 atttctgccc gattttacaa tccatatcaa tttccaacag ctgcctataa aatagttttg
     1621 tccctgtatg tgagcactga aacagcattt ggttgacaca tctagttttt catcttgcag
     1681 tttcaaatcc ttctttttga aaattggatt ttaaaaaaaa gaagtaaaag tcacaccttc
     1741 agggtgttct ttcttgtggc ttgaaagaca acattgcaaa ggcctgtcta aggataggct
     1801 tgtttgtcca ttgggttata acataatgaa agcattggac agatcgtgtc cccctttgga
     1861 ctcttcagta gaatgctttt actaacgcta attacatgtt ttgattatga atgaactaaa
     1921 atagtggcaa tggccttaac cttaggcctg tctttcctca gcctgaatgt gcttttgaat
     1981 ggcacatttc acaccataca ttcataatgc attagcgtta tggccatgat gttgtcatga
     2041 gttttgtatg ggagaaaaaa aatcaattta tcacccattt attatttttt aaccttcgtt
     2101 catgcaagct tattttctac taaaacagtt ttggaattat taaaagcatt gctgatactt
     2161 acttcagata ttatgtctag gctctaagaa tggtttcgac atcctaaaca gccaccaccg
     2221 cggtggcggc cccatatggt cgaggggggg cccgctagat atctcacatg gggggaagtt
     2281 taggaccctc ttgtcttttt gtctgtgtgc atgtatttct ttgtaaagta ctgctatgtt
     2341 tctctttgct gtgtggcaac ttaagcctct tcggcctggg ataaaataat ctgcagtggt
     2401 attaataatg tacataaagt caacatattt gaaagtagat taaaatcctt tttaaatata
     2461 tcaatgatgg caaaaaggtt aaagggggcc taacagtact gtgtgtagtg ttttattttt
     2521 aacagtagta cactataact taaaatagac ttagattaga ctgtttgcat gattatgatt
     2581 ctgtttcctt tatgcatgaa atattgattt tacctttcca gctacttcgt tagctttaat
     2641 tttaaaatta cattaactga gtcttccttc ttgttcgaaa ccagctgttc ctcctcccac
     2701 tgacctgcga ttcaccaaca ttggtccaga caccatgcgt gtcacctggg ctccaccccc
     2761 atccattgat ttaaccaact tcctggtgac cctggccgcc cacctccccg ccgagttcac
     2821 ccctgcggtg cacgcctccc tggacaagtt cctggcttct gtgagcaccg tgctgacctc
     2881 caaataccgt taagctggag cctcggtggc catgcttctt gccccttggg cctcccccca
     2941 gcccctcctc cccttcctgc acccgtaccc ccgtggtctt tgaataaagt ctgagtgggc
     3001 ggcagcctgt gtgtgcctga gttttttccc tcagcaaacg tgccaggcat gggcgtggac
     3061 agcagctggg acacacatgg ctagaacctc tctgcaggaa ttcgatatca agcttatcga
     3121 taccgtcgac ctcgaggggg ggcccggtac c
//
