LOCUS       sequence-82216-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human immunoglobulin gamma 1 antibody expression vector (kappa light
            chain) without variable regions. Enables Colony-PCR screening of 
            false-positive (PCR vector template) colonies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, 
            Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
  TITLE     A tool kit for rapid cloning and expression of recombinant 
            antibodies.
  JOURNAL   Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
  PUBMED    25073855
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-07-30"; volume: "4:5885. doi"; pages: " 
            10.1038/srep05885"
COMMENT     Sequence Label: pVITRO1-dV-IgG1/kappa
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggtgttgtga aaaccaccgc taattcaaag caaccggtat ggactggacc tggaggatcc
       61 tcttcttggt ggcggccgcc acaggcgcgc actccgaatt cgatatcctc gaggctagca
      121 ccaagggccc atcggtcttc cccctggcac cctcctccaa gagcacctct gggggcacag
      181 cggccctggg ctgcctggtc aaggactact tccccgaacc ggtgacggtg tcgtggaact
      241 caggcgccct gaccagcggc gtgcacacct tcccggctgt cctacagtcc tcaggactct
      301 actccctcag cagcgtggtg accgtgccct ccagcagctt gggcacccag acctacatct
      361 gcaacgtgaa tcacaagccc agcaacacca aggtggacaa gaaagttgag cccaaatctt
      421 gtgacaaaac tcacacatgc ccaccgtgcc cagcacctga actcctgggg ggaccgtcag
      481 tcttcctctt ccccccaaaa cccaaggaca ccctcatgat ctcccggacc cctgaggtca
      541 catgcgtggt ggtggacgtg agccacgaag accctgaggt caagttcaac tggtacgtgg
      601 acggcgtgga ggtgcataat gccaagacaa agccgcggga ggagcagtac aacagcacgt
      661 accgtgtggt cagcgtcctc accgtcctgc accaggactg gctgaatggc aaggagtaca
      721 agtgcaaggt ctccaacaaa gccctcccag cccccatcga gaaaaccatc tccaaagcca
      781 aagggcagcc ccgagaacca caggtgtaca ccctgccccc at
//
