LOCUS       sequence-82218-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human immunoglobulin gamma 1 antibody expression vector (kappa light
            chain) without variable regions. Enables Colony-PCR screening of 
            false-positive (PCR vector template) colonies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, 
            Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
  TITLE     A tool kit for rapid cloning and expression of recombinant 
            antibodies.
  JOURNAL   Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
  PUBMED    25073855
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-07-30"; volume: "4:5885. doi"; pages: " 
            10.1038/srep05885"
COMMENT     Sequence Label: pVITRO1-dV-IgG1/kappa
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acgcagtaca aagtgttacc cctctagacc tggaaagacc aggcggagtt tcaagttgca
       61 cgttttgtgt cattggggaa acctgctcct aggcgtacgg gatccctaac actctcccct
      121 gttgaagctc tttgtgacgg gcgagctcag gccctgatgg gtgacttcgc aggcgtagac
      181 tttgtgtttc tcgtagtctg ctttgctcag cgtcagggtg ctgctgaggc tgtaggtgct
      241 gtccttgctg tcctgctctg tgacactctc ctgggagtta cccgattgga gggcgttatc
      301 caccttccac tgtactttgg cctctctggg atagaagtta ttcagcaggc acacaacaga
      361 ggcagttcca gatttcaact gctcatcaga tggcgggaag atgaagacag atggcgccgc
      421 caccgtacgc tcgaggatat cgaattcacc gcggctagct ggaacccaga gcagcagaaa
      481 cccaatgagt tgtgatggca acattccgga ttgctttgaa ttagcggtgg ctttcacaac
      541 acctggaaac gggtttagaa aatacctttg aaccgctaag aggcttgaga attagcctcg
      601 ctccaaactc aaaggggcaa attccaagaa tgacttccag cgccaggctg gcctgactag
      661 tctccaccca ccaaatgtga acaaactcca acgccattag atcccctccc cccgccgcga
      721 cgagcggtgc tcaagagaag cccgaggtga ctatgcggcc gatagaccac ggggtcacag
      781 gaagcagcgg ccggcgaggg gcaaggccct cttcctttgt gtgggt
//
