LOCUS       sequence-82227-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cph8 under a modified PLtetO-1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
  TITLE     Characterizing bacterial gene circuit dynamics with optically 
            programmed gene expression signals.
  JOURNAL   Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
  PUBMED    24608181
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884."
COMMENT     Sequence Label: pCph8
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..17
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tcattaaaga ggagaaaggt acccatggcc accaccgtac aactcagcga ccaatccctc
       61 cgtcagctag aaaccctcgc catccacacc gcccacctga ttcagcccca cggtttagtg
      121 gtggtcctgc aggaaccaga cctcaccatc agccaaatta gcgccaactg caccggcatt
      181 ttagggcgat cgccagagga tttgttgggc agaaccctag gggaagtgtt tgatagcttt
      241 cagattgatc ccatccagag tcgcctaacg gccggacaaa tcagcagcct caaccccagt
      301 aaactttggg cgcgggtcat gggggacgac tttgtcattt ttgacggggt ttttcatcgc
      361 aacagtgacg gtttattggt atgtgaactc gagccagcct acacttccga taatctgccc
      421 ttcctcggtt tttatcacat ggccaacgct gccctgaatc ggttgcgcca acaagctaat
      481 ctacgggatt tctacgatgt tattgtcgaa gaagtccgcc gtatgactgg ctttgaccgg
      541 gtgatgctat accgctttga tgaaaataac cacggtgatg tcattgccga agataaacgg
      601 gatgatatgg aaccctattt gggcctgcac tatcccgaat cggatattcc ccaacccgcc
      661 cgtcggctat ttatccacaa ccccattcga gtaattcccg atgtttatgg tgtggcggtg
      721 cccctgaccc cagcggttaa ccccagcacc aaccgagcgg tggatttaac agaatccatt
      781 ctgcgcagtg cgtaccattg ccacttgacc tatctgaaaa atatgggggt aggagcgtct
      841 ttaaccattt ccctaattaa ggacggccat ctctgggggc tcattgcctg ccaccatcaa
      901 acccccaaag taattccctt tgaactgcgt aaagcctgcg
//
