LOCUS       sequence-82232-e        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector has been discontinued as of 5/8/2014. See Comments below
            for information on the updated version of the lentiCRISPR vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen TS, 
            Heckl D, Ebert BL, Root DE, Doench JG, Zhang F
  TITLE     Genome-scale CRISPR-Cas9 knockout screening in human cells.
  JOURNAL   Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. 
            Epub 2013 Dec 12.
  PUBMED    24336571
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1247005"; journalName: "Science"; date: "2014-01-3-
            3"; volume: "343"; issue: "6166"; pages: "84-7"
COMMENT     Sequence Label: lentiCRISPR
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        71..297
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             298..478
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    525..650
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
       61 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
      121 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
      181 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
      241 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
      301 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      361 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      421 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      481 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      541 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      601 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      661 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      721 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
      781 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
      841 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
      901 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
      961 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1021 atgagggaca attggagaag tgaattatat aaat
//
