LOCUS       sequence-82249-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP and Synaptophysin-TagRFP-T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-N-P-M-EGFP-SynPhRFP-L
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      216..225
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(222..224,225..227,228..740)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(267..288)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(528..547)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atcagattat atcccgcaaa tttatcactt gtttacctct ggaggagaga acatatgggc
       61 tcaactccaa cccttgggag caatataaca aaaaacatgt tatggtgcca ttaaaccgct
      121 gcatttcatc aaagtcaagt tgattacctt tacattttga tcctcttgga tgtgaaaaaa
      181 actattaaca tccctcaaaa gaccccggga aagccgccac catggtgagc aagggcgagg
      241 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      301 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      361 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      421 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      481 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      541 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      601 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      661 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
      721 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      781 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctga
//
