LOCUS       sequence-8226-ex        7452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Chih B, Gollan L, Scheiffele P
  TITLE     Alternative splicing controls selective trans-synaptic interactions 
            of the neuroligin-neurexin complex.
  JOURNAL   Neuron. 2006 Jul 20. 51(2):171-8.
  PUBMED    16846852
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2006 Jul 20. 51(2):171-8."
COMMENT     Sequence Label: pCAG-NL1A
FEATURES             Location/Qualifiers
     source          1..7452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        368..645
                     /label=chicken beta-actin promoter
     intron          647..1664
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1588..1610
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1907..1933
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      2389..2398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4332..4341
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4456..4475)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4521..4576
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4575..4594)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4937..4953)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4937..4953)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4950..4972)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4961..4977
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4985..4991,4992..5009,5010..5015))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5030..5051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5110..5305
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5156..5291
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5311..5445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5348..5367)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5402..5421
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5513..5530)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5684..6272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5764..5783)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6443..7234,7235..7303))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7066..7085
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7304..7408)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(7439..7452,1..366)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      481 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      541 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac
      661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
      721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
      781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
      841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
      901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
      961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctctgg gcacgtgctg
     1681 gttattgtgc tgtctcatca ttttggcaaa gaattcgcgg ccgctagcgc taccggactc
     1741 agatctcgag ctcaagcttg ggaccatggc acttcccaga tgcatgtggc caaattatgt
     1801 ttggagagct atgatggcat gtgtggtcca caggggatcc ggtgccccat tgactctctg
     1861 cttgttggga tgtttgctac agacttttca cgtactctct caaaagtatc catacgacgt
     1921 tccggactac gcattggatg atgtagaccc attggttact actaactttg gcaagattag
     1981 gggaattaag aaagaactca ataatgaaat tttgggtcct gtcattcagt ttcttggggt
     2041 tccatatgcc gctccaccaa caggagaaca tcgtttccag cctccagaac caccatctcc
     2101 ctggtctgac atccggaacg ccactcagtt tgctcctgta tgtccccaga atatcattga
     2161 tggcagattg cctgaagtta tgcttcctgt gtggttcact aataacttgg atgtggtttc
     2221 atcatacgtc caagaccaga gtgaagactg tctatactta aacatctatg tcccaactga
     2281 agatggtccc cttacaaaga aacacacaga tgatttaggt gataatgacg gtgctgaaga
     2341 tgaagatatt cgggacagtg ggggtcccaa accagtgatg gtgtacatcc atggcggctc
     2401 ttacatggaa ggtactggaa atctgtatga tgggagtgtc ttggcaagct atggcaatgt
     2461 gatcgtcatc acagtcaact atcggcttgg ggtacttggc ttcttgagca caggggatca
     2521 ggctgccaaa ggaaactacg ggctccttga cctcatccag gccctaagat ggaccagcga
     2581 gaacattggg ttctttggtg gtgacccctt gcgaatcact gtgtttggat caggcgctgg
     2641 gggttcatgt gtcaacctgc tgactttatc ccattattct gaaggacttt ttcaacgagc
     2701 aatagctcag agtggaacag ccctttccag ctgggctgtt agtttccagc ctgcaaaata
     2761 cgctagaatt ctggccacaa aagttggctg caatgtttca gatacagtag agttagtaga
     2821 atgcctgcag aagaagcctt acaaagaact tgttgatcaa gatgttcaac cagcccgata
     2881 ccacatagcc tttggacctg tgatcgatgg tgatgtaata ccagatgacc ctcagatact
     2941 gatggaacaa ggagagttcc tcaactatga tataatgttg ggagttaacc aaggggaagg
     3001 gttgaagttt gtcgaaaata tagtagatag tgatgatggt gtatcagcca gtgatttcga
     3061 ctttgctgtt tctaattttg ttgataattt atatggatat cctgaaggca aagatgtttt
     3121 gagagaaacc attaaattca tgtatactga ctgggctgat cgccataatc ctgaaactag
     3181 aaggaagaca ttgttggctt tgtttacgga ccatcaatgg gtagcacctg ctgtggccac
     3241 agcagacctt cactcgaact ttggctcacc tacatacttc tatgcctttt atcatcattg
     3301 ccaaacagat caagttccag cttgggctga tgcagctcat ggggatgagg ttccctatgt
     3361 gttgggaatc cccatgattg gccctacaga gttatttcct tgcaatttct ccaagaatga
     3421 tgtgatgttg agtgcagtag taatgacata ctggacgaat tttgctaaaa ctggtgaccc
     3481 aaatcaacca gttcctcaag acacaaaatt catccatacc aaacccaacc gctttgaaga
     3541 agtagcatgg accagatatt cccagaaaga ccagctttat ctccatattg gattaaaacc
     3601 gagagttaaa gagcattaca gagccaataa ggtaaatctc tggctggagc tggtacctca
     3661 tctgcataat ctcaatgaca tttctcagta tacctcgaca acaactaaag tgccatccac
     3721 ggacatcact ctcagaccta caaggaagaa ttccacacca gtcacatcag cctttcccac
     3781 tgccaaacag gatgatccca agcaacaacc aagccccttc tcggtggatc agagggacta
     3841 ctccacagag ctaagtgtca ctatcgcagt gggggcctct ctgctgtttc tcaacatctt
     3901 ggcttttgca gccctgtact acaagaagga taagaggaga catgatgtcc accggaggtg
     3961 cagccctcag cgcacgacca ccaacgacct aacccatgct ccagaagagg aaattatgtc
     4021 tctccaaatg aagcacactg acttggatca cgagtgtgag tccatccatc cacatgaggt
     4081 ggttcttcgg accgcctgtc ccccagatta tactctagct atgaggaggt cacctgatga
     4141 tattccacta atgacaccta acaccatcac aatgattccc aacactatac cagggattca
     4201 gcccttacat acattcaaca catttactgg aggacagaat aatacactgc cccatcccca
     4261 cccacacccc cattcacatt caacaaccag ggtatagcca gatgagagag acaaactgat
     4321 taatcccgcg gccatggcgg ccgggagcat gcgacgtcgg gcccggggag ctcaattcac
     4381 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     4441 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     4501 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     4561 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     4621 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     4681 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     4741 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     4801 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     4861 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     4921 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4981 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     5041 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5101 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5161 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5221 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5281 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
     5341 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5401 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
     5461 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5521 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5581 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5641 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5701 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5761 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5821 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5881 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5941 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6001 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6061 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6121 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6181 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6241 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6301 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6361 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6421 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6481 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6541 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6601 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6661 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6721 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6781 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6841 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6901 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6961 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7021 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7081 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7141 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7201 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7261 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7321 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7381 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
     7441 cattgattat tg
//
