LOCUS       sequence-82264-e         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the
            linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI 
            /ClaI). Plasmid confers ampicillin resistance to DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: p416GAL1-Zipp.-L-IFP-F[1] (Yeast)
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..129
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 atgaacactg aagccgccag gcgttctcgt gcgagaaagt tgcaaagaat gaaacaactt
       61 gaagacaagg ttgaagaatt gctttcgaaa aattatcact tggaaaatga ggttgccaga
      121 ttaaagaaat tagttggcga acgcatcgat ggtggcggtg gctctggagg tggtgggtcc
      181 tccggagctc gggaccctct gccattcttt ccacctctgt acctgggcgg ccctgagatt
      241 acaaccgaga actgcgagag agagcctatc cacattcctg ggtccatcca gccacacggg
      301 gctctgctca cagctgacgg ccactccgga gaggtgctcc aagtgtccct gaatgccgct
      361 accttcctgg gccaggagcc tactgtgctg cgggggcaga ccctggctgc cctgctcccc
      421 gagcagtggc cagccctgca ggcagccctg cccccaggat gtccagatgc cctccaatac
      481 agggccaccc tcgactggcc agctgctggg cacctcagcc tgactgtgca tcgggtggct
      541 gaactcctga tcctggagtt cgaacctacc gaggcctggt aa
//
